Literature DB >> 28779838

The MEMbrane Protein Single ShoT Amplification Recipe: MemStar.

Abdul Aziz-Qureshi1, Pascal F Meier1, Chiara Lee1, David Drew2.   

Abstract

Here, we present a simple overexpression condition for high-throughput screening of membrane proteins in Escherichia coli. For the vast majority of bacterial membrane protein targets tested the MEMbrane protein Single shoT Amplification Recipe-MemStar-leads to high production yields of target protein. The use of MemStar has facilitated structural studies of several transport proteins.
© 2017 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  E. coli; High throughput; Membrane protein; Overexpression; Structure

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Year:  2017        PMID： 28779838     DOI： 10.1016/bs.mie.2017.05.010

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  2 in total

1.  Thermostability-based binding assays reveal complex interplay of cation, substrate and lipid binding in the bacterial DASS transporter, VcINDY.

Authors:  Connor D D Sampson; Cristina Fàbregas Bellavista; Matthew J Stewart; Christopher Mulligan
Journal:  Biochem J       Date:  2021-11-12       Impact factor: 3.857

2.  Solvent accessibility changes in a Na+-dependent C4-dicarboxylate transporter suggest differential substrate effects in a multistep mechanism.

Authors:  Connor D D Sampson; Matthew J Stewart; Joseph A Mindell; Christopher Mulligan
Journal:  J Biol Chem       Date:  2020-10-21       Impact factor: 5.157
  2 in total

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