| Literature DB >> 2877922 |
F K Lin, C H Lin, P H Lai, J K Browne, J C Egrie, R Smalling, G M Fox, K K Chen, M Castro, S Suggs.
Abstract
The erythropoietin (Epo) gene from Cynomolgus monkeys has been isolated from a kidney cDNA library using mixed 20-mer oligodeoxynucleotide probes. The gene encodes a 168 amino acid (aa) mature protein with a calculated Mr of 18,490 and a presumptive signal peptide of 24 aa. The Epo gene, when transfected into Chinese hamster ovary (CHO) cells, produces a glycosylated protein with an apparent Mr of 34,000. The expressed product is biologically active in vivo. The monkey gene exhibits 92% and 94% homology to the human gene at the aa and nucleotide sequence levels, respectively. When compared with the human Epo, monkey Epo has an additional 3-aa residue at the N terminus of the mature protein and a deletion of an internal lysine residue.Entities:
Mesh：
Substances：
Year: 1986 PMID： 2877922 DOI： 10.1016/0378-1119(86)90183-6
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688