Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Lipoprotein Receptor-related Protein 6 Signaling is Necessary for Vasculogenic Differentiation of Human Dental Pulp Stem Cells.

Literature DB >> 28778505

Lipoprotein Receptor-related Protein 6 Signaling is Necessary for Vasculogenic Differentiation of Human Dental Pulp Stem Cells.

Gleyce O Silva¹, Zhaocheng Zhang², Carolina Cucco², Min Oh², Carlos H R Camargo³, Jacques E Nör⁴.

Abstract

The aim of this study was to evaluate the effects of Wnt signaling through lipoprotein receptor-related protein 6 (LRP6) and Frizzled6 on the endothelial differentiation of dental pulp stem cells (DPSCs). DPSCs were stably transduced with enhanced green fluorescent protein (EGFP)-tagged lentiviral vectors (short hairpin RNA-LRP6, short hairpin RNA-Frizzled6, or empty vector controls). We evaluated the effects of LRP6 and Frizzled6 on expression of endothelial markers and on capillary tube formation mediated by DPSCs induced with recombinant human Wnt1 (rhWnt1) and/or recombinant human vascular endothelial growth factor165 (rhVEGF165). In vivo, tooth slices/scaffolds were seeded with LRP6-silenced, Frizzled6-silenced, or vector control DPSC cells and transplanted into immunodeficient mice. The density of blood vessels generated by DPSCs differentiated into vascular endothelial cells was analyzed by immunohistochemistry for EGFP. The rhWnt1 and rhVEGF165 induced expression of active β-catenin in control DPSCs and in Frizzled6-silenced DPSCs, but not in LRP6-silenced DPSCs. Furthermore, VEGF and interleukin-8 were downregulated in LRP6-silenced DPSCs, but not in control DPSCs or in Frizzled6-silenced DPSCs (P < .05). Likewise, rhWnt1 and rhVEGF165 induced expression of the endothelial marker VEGF receptor-2 in control DPSCs and in Frizzled6-silenced DPSCs, but not in LRP6-silenced DPSCs. These data correlated with a trend for lower density of capillary sprouts generated by LRP6-silenced DPSCs when compared with control DPSCs in Matrigel. In vivo, tooth slice/scaffolds seeded with DPSC-short hairpinRNA-LRP6 cells showed lower density of human blood vessels (ie, EGFP-positive blood vessels), when compared with tooth slice/scaffolds seeded with vector control cells (P < .05). Collectively, these data demonstrated that LRP6 signaling is necessary for the vasculogenic differentiation of human DPSCs.

Entities: CellLine Chemical Disease Gene Species

Keywords: Angiogenesis; Wnt; cell fate; regenerative endodontics; tissue engineering

Mesh：

Substances：

Year: 2017 PMID： 28778505 PMCID： PMC5657009 DOI： 10.1016/j.joen.2017.06.006

Source DB: PubMed Journal: J Endod ISSN： 0099-2399 Impact factor: 4.171

31 in total

1. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.

Authors: S Gronthos; M Mankani; J Brahim; P G Robey; S Shi
Journal: Proc Natl Acad Sci U S A Date: 2000-12-05 Impact factor: 11.205

2. Stem cell properties of human dental pulp stem cells.

Authors: S Gronthos; J Brahim; W Li; L W Fisher; N Cherman; A Boyde; P DenBesten; P Gehron Robey; S Shi
Journal: J Dent Res Date: 2002-08 Impact factor: 6.116

3. SHED differentiate into functional odontoblasts and endothelium.

Authors: V T Sakai; Z Zhang; Z Dong; K G Neiva; M A A M Machado; S Shi; C F Santos; J E Nör
Journal: J Dent Res Date: 2010-04-15 Impact factor: 6.116

4. Wnt/β-catenin pathway regulates bone morphogenetic protein (BMP2)-mediated differentiation of dental follicle cells.

Authors: K G Silvério; K C Davidson; R G James; A M Adams; B L Foster; F H Nociti; M J Somerman; R T Moon
Journal: J Periodontal Res Date: 2011-12-11 Impact factor: 4.419

5. Wnt/β-Catenin Signaling Determines the Vasculogenic Fate of Postnatal Mesenchymal Stem Cells.

Authors: Zhaocheng Zhang; Felipe Nör; Min Oh; Carolina Cucco; Songtao Shi; Jacques E Nör
Journal: Stem Cells Date: 2016-03-11 Impact factor: 6.277

6. Endothelial differentiation of SHED requires MEK1/ERK signaling.

Authors: L W Bento; Z Zhang; A Imai; F Nör; Z Dong; S Shi; F B Araujo; J E Nör
Journal: J Dent Res Date: 2012-10-31 Impact factor: 6.116

7. A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin.

Authors: P D McCrea; C W Turck; B Gumbiner
Journal: Science Date: 1991-11-29 Impact factor: 47.728

8. Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors.

Authors: Silvia Maretto; Michelangelo Cordenonsi; Sirio Dupont; Paola Braghetta; Vania Broccoli; A Bassim Hassan; Dino Volpin; Giorgio M Bressan; Stefano Piccolo
Journal: Proc Natl Acad Sci U S A Date: 2003-03-07 Impact factor: 11.205

9. Angiogenic properties of human dental pulp stem cells.

Authors: Annelies Bronckaers; Petra Hilkens; Yanick Fanton; Tom Struys; Pascal Gervois; Constantinus Politis; Wendy Martens; Ivo Lambrichts
Journal: PLoS One Date: 2013-08-07 Impact factor: 3.240

10. AKT activation by N-cadherin regulates beta-catenin signaling and neuronal differentiation during cortical development.

Authors: Jianing Zhang; Julie R Shemezis; Erin R McQuinn; Jing Wang; Maria Sverdlov; Anjen Chenn
Journal: Neural Dev Date: 2013-04-25 Impact factor: 3.842

4 in total