Jeong-Ki Jo1, Ahmed El-Fiqi2, Jung-Hwan Lee3, Dong-Ae Kim1, Hae-Won Kim4, Hae-Hyoung Lee5. 1. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea. 2. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Glass Research Department, National Research Center, Cairo 12622, Egypt. 3. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea. Electronic address: ducious@gmail.com. 4. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine Research Center, Dankook University, Cheonan. 5. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea; Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea. Electronic address: haelee@dku.edu.
Abstract
OBJECTIVES: Even though polymethyl methacrylate (PMMA) resin is widely used as a dental material, it has poor microbial anti-adhesive properties, which accelerates oral infections. In this investigation, silver-sulfadiazine (AgSD)-loaded mesoporous silica nanoparticles (Ag-MSNs) were incorporated into PMMA to introduce long-term microbial anti-adhesive effects and to make PMMA a rechargeable resin. METHODS: After characterization of the Ag-MSNs in terms of their mesoporous characteristics and drug loading capacity, the 3 point flexural test and hardness were evaluated in PMMA incorporating Ag-MSNs (0.5, 1, 2.5 and 5%). Anti-adhesive effects were observed for Candida albicans and Streptococcus oralis with experimental specimens for up to 28days and after recharging with AgSD. RESULTS: A typical spherical morphology and high mesoporosity were observed for the MSNs used for loading AgSD. Incorporation of Ag-MSNs into PMMA (0.5, 1, 2.5 and 5%) sustained its flexural strength but increased its surface hardness. Anti-adhesive effects were observed after 1h of exposure to both microbial species, and the effects accelerated with increasing Ag-MSN incorporation into PMMA. Long-term microbial anti-adhesive effects were observed for up to 14 days, and further long-term (7 days) anti-adhesive effects were observed after reloading the Ag-MSN-incorporated PMMA (aged for 28 days) with AgSD; these effects were largely caused by released silver ions and partially by changes in surface hydrophilicity. No cytotoxicity to keratinocytes was observed. CONCLUSIONS: The improved mechanical properties and the prolonged microbial anti-adhesive effects, which lasted after reloading of the drug, suggest the potential usefulness of Ag-MSN-incorporated PMMA as a microbial anti-adhesive dental material. SIGNIFICANCE: Ag-MSN-incorporated PMMA can be used as a microbial anti-adhesive dental material for dentures, orthodontic devices and provisional restorative materials.
OBJECTIVES: Even though polymethyl methacrylate (PMMA) resin is widely used as a dental material, it has poor microbial anti-adhesive properties, which accelerates oral infections. In this investigation, silver-sulfadiazine (AgSD)-loaded mesoporous silica nanoparticles (Ag-MSNs) were incorporated into PMMA to introduce long-term microbial anti-adhesive effects and to make PMMA a rechargeable resin. METHODS: After characterization of the Ag-MSNs in terms of their mesoporous characteristics and drug loading capacity, the 3 point flexural test and hardness were evaluated in PMMA incorporating Ag-MSNs (0.5, 1, 2.5 and 5%). Anti-adhesive effects were observed for Candida albicans and Streptococcus oralis with experimental specimens for up to 28days and after recharging with AgSD. RESULTS: A typical spherical morphology and high mesoporosity were observed for the MSNs used for loading AgSD. Incorporation of Ag-MSNs into PMMA (0.5, 1, 2.5 and 5%) sustained its flexural strength but increased its surface hardness. Anti-adhesive effects were observed after 1h of exposure to both microbial species, and the effects accelerated with increasing Ag-MSN incorporation into PMMA. Long-term microbial anti-adhesive effects were observed for up to 14 days, and further long-term (7 days) anti-adhesive effects were observed after reloading the Ag-MSN-incorporated PMMA (aged for 28 days) with AgSD; these effects were largely caused by released silver ions and partially by changes in surface hydrophilicity. No cytotoxicity to keratinocytes was observed. CONCLUSIONS: The improved mechanical properties and the prolonged microbial anti-adhesive effects, which lasted after reloading of the drug, suggest the potential usefulness of Ag-MSN-incorporated PMMA as a microbial anti-adhesive dental material. SIGNIFICANCE: Ag-MSN-incorporated PMMA can be used as a microbial anti-adhesive dental material for dentures, orthodontic devices and provisional restorative materials.
Authors: Hang-Nga Mai; Do-Yeon Kim; Dong Choon Hyun; Ju Hayng Park; Sang Min Lee; Du-Hyeong Lee Journal: J Clin Med Date: 2019-11-01 Impact factor: 4.241