Evaluation of blood monocyte and lymphocyte population in broiler chicken after vaccination and experimental challenge with Newcastle disease virus.

In the present study, after vaccination and challenge with Newcastle disease virus, changes in the population of blood monocytes and lymphocytes of broiler chickens were evaluated using flow cytometry. 300 apparently healthy 1-day-old Cobb broiler chicks were divided randomly into four experimental groups (n=75). At 20days of age the chicks in group 1 and 2 were vaccinated with live B1 ND vaccine. Those in group 2 were additionally injected with a killed vaccine simultaneously and group 3 chicks received only the adjuvant of the killed vaccine. The birds in groups 1, 2 and 3 were challenged with a velogenic ND virus and those in group 4 were treated as control. Sampling was done on days 1,2,3,7 after vaccination and also on 1, 2, 3,7,14, 21 post challenge days. In this study percentage of B cell population was increased after vaccination and challenge in vaccinated birds, but CD3+ cells were decreased after vaccination and challenge, which showed B cells have more expansion than T cells. The CD4+ cell percentage in vaccinated birds was always lower than control birds. However, the percentage of CD8+ cells in vaccinated birds was increased. Results indicate increased CMI with the live NDV vaccination. In this study CD4/CD8 ratio in control birds was about 1.5 at 30days of age and it was slightly lower in vaccinated and challenged birds. The percentage of monocytes in vaccinated birds was significantly higher than control birds from 3days post vaccination to the end of the experiment.