Armin Mooranian1, Rebecca Negrulj1, Ryu Takechi2, Emma Jamieson3,4, Grant Morahan3,4, Hani Al-Salami1. 1. a Biotechnology and Drug Development Research Laboratory, School of Pharmacy , Curtin Health Innovation Research Institute, Curtin University , Perth , WA , Australia. 2. b School of Public Health , Curtin Health Innovation Research Institute, Curtin University , Perth , WA , Australia. 3. c Centre for Medical Research , University of Western Australia , Perth , WA , Australia. 4. d Clinical Sciences Division , The Harry Perkins Institute of Medical Research , Perth , WA , Australia.
Abstract
INTRODUCTION: Current trials for β-cell transplantation have been hindered by poor cell viability and function post-transplantation. Recently, electric charges of the microencapsulating formulation carrying β-cells have shown significant effects on cell survival and function. Thus, this study aimed at investigating the effects of electric charge, of novel colloidal formulation containing β-cells, on cell viability, biological activity and insulin release. METHODS: A new formulation, containing high ratios of poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone (1:1:0.1 ratio), was used to form microcapsules utilizing 800 V and 2000 Hz encapsulating conditions. The bile acid, ursodeoxycholic acid, was added into the microcapsules to measure its effects on electric charges. RESULTS: The electric charge of the microencapsulating formulation was enhanced by bile acid addition, and resulted in better cell viability and function. CONCLUSION: Ursodeoxycholic acid microencapsulated with poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone at 1:1:0.1 ratio, using 800 V and 2000 Hz microencapsulating conditions, produced enhanced electrokinetic parameters of microcapsules with optimized cell functions. This suggests that electric charge of formulations containing pancreatic β-cell may have significant effects on cell mass and functions, post-transplantation.
INTRODUCTION: Current trials for β-cell transplantation have been hindered by poor cell viability and function post-transplantation. Recently, electric charges of the microencapsulating formulation carrying β-cells have shown significant effects on cell survival and function. Thus, this study aimed at investigating the effects of electric charge, of novel colloidal formulation containing β-cells, on cell viability, biological activity and insulin release. METHODS: A new formulation, containing high ratios of poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone (1:1:0.1 ratio), was used to form microcapsules utilizing 800 V and 2000 Hz encapsulating conditions. The bile acid, ursodeoxycholic acid, was added into the microcapsules to measure its effects on electric charges. RESULTS: The electric charge of the microencapsulating formulation was enhanced by bile acid addition, and resulted in better cell viability and function. CONCLUSION:Ursodeoxycholic acid microencapsulated with poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone at 1:1:0.1 ratio, using 800 V and 2000 Hz microencapsulating conditions, produced enhanced electrokinetic parameters of microcapsules with optimized cell functions. This suggests that electric charge of formulations containing pancreatic β-cell may have significant effects on cell mass and functions, post-transplantation.
Authors: Gabriel Alexander Salg; Nathalia A Giese; Miriam Schenk; Felix J Hüttner; Klaus Felix; Pascal Probst; Markus K Diener; Thilo Hackert; Hannes Götz Kenngott Journal: J Tissue Eng Date: 2019-10-30 Impact factor: 7.813
Authors: Armin Mooranian; Thomas Foster; Corina M Ionescu; Louise Carey; Daniel Walker; Melissa Jones; Susbin Raj Wagle; Bozica Kovacevic; Jacqueline Chester; Edan Johnstone; Jafri Kuthubutheen; Daniel Brown; Marcus D Atlas; Momir Mikov; Hani Al-Salami Journal: Drug Des Devel Ther Date: 2021-10-23 Impact factor: 4.162