Literature DB >> 28772

Characterization of sterol-ester hydrolase in Saccharomyces cerevisiae.

S Taketani, T Osumi, H Katsuki.   

Abstract

A homgenate of Saccharomyces cerevisiae grown under semi-anaerobic as well as aerobic conditions was found to catalyze the hydrolysis of fatty acid esters of sterols in the presence of Triton X-100. The enzyme levels in cells grown under various conditions were similar and the enzyme had a broad substrate specificity for sterol esters. The enzyme was localized in the mitochondrial fraction for the aerobically grown cells and in the mitochondrial and cytosolic fractions for the semi-anaerobically grown cells.

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Year:  1978        PMID: 28772     DOI: 10.1016/0005-2744(78)90202-4

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  7 in total

1.  The Saccharomyces cerevisiae YLL012/YEH1, YLR020/YEH2, and TGL1 genes encode a novel family of membrane-anchored lipases that are required for steryl ester hydrolysis.

Authors:  René Köffel; Rashi Tiwari; Laurent Falquet; Roger Schneiter
Journal:  Mol Cell Biol       Date:  2005-03       Impact factor: 4.272

2.  Uptake and interconversion of cholesterol and cholesteryl esters byPhytophthora cactorum.

Authors:  C G Elliott; B A Knights
Journal:  Lipids       Date:  1981-01       Impact factor: 1.880

3.  Sterol composition of yeast organelle membranes and subcellular distribution of enzymes involved in sterol metabolism.

Authors:  E Zinser; F Paltauf; G Daum
Journal:  J Bacteriol       Date:  1993-05       Impact factor: 3.490

4.  Sterol ester hydrolase in Fusarium oxysporum.

Authors:  C Madhosingh; W Orr
Journal:  Lipids       Date:  1981-02       Impact factor: 1.880

5.  Primary site of action of ketoconazole on Candida albicans.

Authors:  J Uno; M L Shigematsu; T Arai
Journal:  Antimicrob Agents Chemother       Date:  1982-06       Impact factor: 5.191

6.  Purification, characterization, and molecular cloning of organic-solvent-tolerant cholesterol esterase from cyclohexane-tolerant Burkholderia cepacia strain ST-200.

Authors:  Yasuhiko Takeda; Rikizo Aono; Noriyuki Doukyu
Journal:  Extremophiles       Date:  2006-02-07       Impact factor: 2.395

7.  The Chlamydia trachomatis CT149 protein exhibits esterase activity in vitro and catalyzes cholesteryl ester hydrolysis when expressed in HeLa cells.

Authors:  Jan Peters; Vijaya Onguri; Satoru K Nishimoto; Tony N Marion; Gerald I Byrne
Journal:  Microbes Infect       Date:  2012-08-23       Impact factor: 2.700

  7 in total

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