Enzyme immunoassay with cell suspensions: studies on reactions between serum antibodies and cell-surface antigens.

The following reactions of serum antibodies with cell surface antigens were studied by means of an enzyme immunoassay with cell suspensions: Rh antiserum with human red blood cells; H-2 antisera with murine red blood cells; H-2 antisera with murine thymocytes and hepatocytes; Thy-1 antiserum with murine thymocytes; polyvalent and monovalent HLA alloantisera with human leukocytes and human mononuclear cells, and antisera of murine origin with human and marmoset lymphoid cell lines. In all instances, with the exception of monovalent HLA antisera, the assay proved to be a sensitive and highly specific procedure.