| Literature DB >> 28769375 |
P Sivadas1, Harsh Kumar2, C Lakshmanan3, J R Bhardwaj4.
Abstract
A rapid method for obtaining high quality paraffin sections is described. This method enables preparation of paraffin blocks in about 20 minutes and is based on microwave stimulated tissue fixation and processing. A total of 200 tissues were processed. Tissue blocks of size 1×1×0.5 were found ideal for processing. Three protocols were evaluated for tissue processing using different timings of exposure to graded alcohols, chloroform and wax. It was observed that utilizing an additional step of exposure to 70 per cent alcohol during tissue dehydration resulted in preparation of paraffin blocks which were light microscopically excellent and highly comparable to conventional methods.Entities:
Keywords: Histopathology; Microwaves; Tissue fixation
Year: 2017 PMID： 28769375 PMCID： PMC5530403 DOI： 10.1016/S0377-1237(17)30791-8
Source DB: PubMed Journal: Med J Armed Forces India ISSN： 0377-1237