Tomer Illouz1, Ravit Madar2, Kathleen Griffioen3, Eitan Okun4. 1. The Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan, 5290002, Israel. 2. The Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan, 5290002, Israel; The Mina and Everard Goodman faculty of Life sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel. 3. Department of Biology and Chemistry, Liberty University, Lynchburg, VA 24515, USA. 4. The Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan, 5290002, Israel; The Mina and Everard Goodman faculty of Life sciences, Bar-Ilan University, Ramat Gan, 5290002, Israel. Electronic address: eitan.okun@biu.ac.il.
Abstract
BACKGROUND: Amyloid-β (Aβ), a hallmark of Alzheimer's disease (AD), has long been a focus of basic and translation research in AD. Quantification and dissociation of the Aβ fractions in their soluble and insoluble forms, is a key factor in numerous AD studies. NEW METHOD: Here we provide a generalized sandwich-enzyme-linked-immuno-sorbent-assay (sELISA) protocol for quantification of human and murine Aβ1-40 and Aβ1-42 and dissociation of these peptides to their soluble-oligomeric and insoluble-fibrillar forms. RESULTS: We have validated the levels of soluble and insoluble Aβ1-40 and Aβ1-42 in the 5XFAD AD and the Ts65Dn Down-Syndrome (DS) mouse models in both the cortex, hippocampus and blood as follows: (1) blood levels of Aβ1-40 and Aβ1-42 are elevated in both mouse strains. (2) 5XFAD mice exhibit elevated soluble and insoluble Aβ1-40 in cortical and hippocampal tissues, soluble Aβ1-42 in the hippocampus, and insoluble Aβ1-42in both cortical and hippocampal tissues (3) Ts65Dn mice exhibit elevated levels of Aβ1-40 in the cortex. COMPARISON WITH EXISTING METHODS: Several methodologies have been proposed for the high throughput measure of Aβ, including HPLC-mass-spectrometry, micro-immunoelectrodes, immunoprecipitation and ELISA. Although commercial sELISA kits are widely used, herein, we describe a more accessible and cost-effective in-house protocol enabling to measure either human or murine, soluble and insoluble Aβ1-40 and Aβ1-42 levels. CONCLUSIONS: We provide a streamlined and accessible protocol for the assessment of soluble and insoluble Aβ1-40 and Aβ1-42 levels from mouse or human origins, enabling a higher accessibility for researchers in the field to generate reliable Aβ-related measurements.
BACKGROUND: Amyloid-β (Aβ), a hallmark of Alzheimer's disease (AD), has long been a focus of basic and translation research in AD. Quantification and dissociation of the Aβ fractions in their soluble and insoluble forms, is a key factor in numerous AD studies. NEW METHOD: Here we provide a generalized sandwich-enzyme-linked-immuno-sorbent-assay (sELISA) protocol for quantification of human and murine Aβ1-40 and Aβ1-42 and dissociation of these peptides to their soluble-oligomeric and insoluble-fibrillar forms. RESULTS: We have validated the levels of soluble and insoluble Aβ1-40 and Aβ1-42 in the 5XFAD AD and the Ts65Dn Down-Syndrome (DS) mouse models in both the cortex, hippocampus and blood as follows: (1) blood levels of Aβ1-40 and Aβ1-42 are elevated in both mouse strains. (2) 5XFAD mice exhibit elevated soluble and insoluble Aβ1-40 in cortical and hippocampal tissues, soluble Aβ1-42 in the hippocampus, and insoluble Aβ1-42in both cortical and hippocampal tissues (3) Ts65Dnmice exhibit elevated levels of Aβ1-40 in the cortex. COMPARISON WITH EXISTING METHODS: Several methodologies have been proposed for the high throughput measure of Aβ, including HPLC-mass-spectrometry, micro-immunoelectrodes, immunoprecipitation and ELISA. Although commercial sELISA kits are widely used, herein, we describe a more accessible and cost-effective in-house protocol enabling to measure either human or murine, soluble and insoluble Aβ1-40 and Aβ1-42 levels. CONCLUSIONS: We provide a streamlined and accessible protocol for the assessment of soluble and insoluble Aβ1-40 and Aβ1-42 levels from mouse or human origins, enabling a higher accessibility for researchers in the field to generate reliable Aβ-related measurements.