Literature DB >> 28766274

Pathogen or contaminant? Distinguishing true infection from synovial fluid culture contamination in patients with suspected septic arthritis.

Mary Louise Fowler1, Clara Zhu2, Kevin Byrne2, Sarah B Lieber3, Andrew Moore4, Robert H Shmerling3, Ziv Paz3.   

Abstract

BACKGROUND: Isolation of bacteria from synovial fluid (SF) is the gold standard for diagnosis of septic arthritis (SA). Contamination results in misdiagnosis and mismanagement. This study identifies clinical characteristics, microbiology, and outcomes of patients with contaminated SF and compares them with patients with true SA.
METHODS: We conducted a retrospective study including all patients aged 18 and older admitted to a single, tertiary-care hospital between 1998 and 2015 with suspected SA and positive SF cultures. Contamination cases were determined by infectious disease specialists involved in the patients' care and a clinical course inconsistent with SA.
RESULTS: 398 patients with true SA and 22 with contaminated SF were identified. The SA group was younger (60.9 vs. 75.6 years; p < 0.01), had higher peripheral polymorphonuclear lymphocytes (78.0 vs. 69.4%; p < 0.01) and SF white blood cell count (91.7 vs. 25.6K/mL; p = 0.02), and longer mean length of stay (10.9 vs. 6.7 days; p = 0.02). The average time to positive culture was longer in the contaminated group (3.62 vs. 1.4 days; p < 0.01). The SA group was less likely to receive a new rheumatologic diagnosis within 1 year (3.0 vs. 36.4%; p < 0.01).
CONCLUSION: This is the first study of its kind looking at clinical features and outcomes of patients with contaminated SF. These patients present with less severe disease, have better outcomes, and receive new rheumatologic diagnoses in more than a third of cases within 1 year. We recommend a conservative approach for patients with suspected contaminated SF, mild symptoms, and no bacterial growth within the first 48 h.

Entities:  

Keywords:  Blood cultures; Coagulase-negative; Contamination; Septic arthritis; Staphylococcus aureus; Synovial fluid culture

Mesh:

Year:  2017        PMID: 28766274     DOI: 10.1007/s15010-017-1051-y

Source DB:  PubMed          Journal:  Infection        ISSN: 0300-8126            Impact factor:   3.553


  14 in total

1.  Relevance of the number of positive bottles in determining clinical significance of coagulase-negative staphylococci in blood cultures.

Authors:  S Mirrett; M P Weinstein; L G Reimer; M L Wilson; L B Reller
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

2.  Minimizing the workup of blood culture contaminants: implementation and evaluation of a laboratory-based algorithm.

Authors:  S S Richter; S E Beekmann; J L Croco; D J Diekema; F P Koontz; M A Pfaller; G V Doern
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

3.  Contaminant blood cultures and resource utilization. The true consequences of false-positive results.

Authors:  D W Bates; L Goldman; T H Lee
Journal:  JAMA       Date:  1991-01-16       Impact factor: 56.272

Review 4.  Current blood culture methods and systems: clinical concepts, technology, and interpretation of results.

Authors:  M P Weinstein
Journal:  Clin Infect Dis       Date:  1996-07       Impact factor: 9.079

5.  Clinical significance of methicillin-resistant coagulase-negative staphylococci obtained from sterile specimens.

Authors:  Masato Tashiro; Koichi Izumikawa; Nobuyuki Ashizawa; Munetoshi Narukawa; Yoshihiro Yamamoto
Journal:  Diagn Microbiol Infect Dis       Date:  2014-09-30       Impact factor: 2.803

6.  High diagnostic value of synovial biopsy in periprosthetic joint infection of the hip.

Authors:  Bernd Fink; Alexander Gebhard; Martin Fuerst; Irina Berger; Peter Schäfer
Journal:  Clin Orthop Relat Res       Date:  2012-07-18       Impact factor: 4.176

Review 7.  Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci.

Authors:  M A Pfaller; L A Herwaldt
Journal:  Clin Microbiol Rev       Date:  1988-07       Impact factor: 26.132

8.  Controlled evaluation of 5 versus 10 milliliters of blood cultured in aerobic BacT/Alert blood culture bottles.

Authors:  M P Weinstein; S Mirrett; M L Wilson; L G Reimer; L B Reller
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948

9.  Effect of iodophor vs iodine tincture skin preparation on blood culture contamination rate.

Authors:  C L Strand; R R Wajsbort; K Sturmann
Journal:  JAMA       Date:  1993-02-24       Impact factor: 56.272

10.  Utility of percutaneous joint aspiration and synovial biopsy in identifying culture-positive infected hip arthroplasty.

Authors:  M Connor Cross; Mark J Kransdorf; F Spencer Chivers; Roxanne Lorans; Catherine C Roberts; Adam J Schwartz; Christopher P Beauchamp
Journal:  Skeletal Radiol       Date:  2013-11-24       Impact factor: 2.199

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  1 in total

1.  Hip Synovial Fluid Cell Counts in Children From a Lyme Disease Endemic Area.

Authors:  Arianna H Dart; Kenneth A Michelson; Paul L Aronson; Aris C Garro; Thomas J Lee; Kimberly M Glerum; Peter A Nigrovic; Mininder S Kocher; Richard G Bachur; Lise E Nigrovic
Journal:  Pediatrics       Date:  2018-04-18       Impact factor: 7.124

  1 in total

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