| Literature DB >> 28763966 |
Wei Wang1, Xi-Wen Fu2, Xin-Long Dai3, Fang Hua4, Gang-Xiu Chu5, Ming-Jie Chu6, Feng-Lin Hu7, Tie-Jun Ling8, Li-Ping Gao9, Zhong-Wen Xie10, Xiao-Chun Wan11, Guan-Hu Bao12.
Abstract
Zijuan tea is a special cultivar of Yunnan broad-leaf tea (Camellia sinensis var. assamica) with purple buds, leaves, and stems. Phytochemical study on this tea led to the discovery of three hydroxycinnamoylated catechins (HCCs) (1-3), seven other catechins (4-10), three proanthocyanidins (11-13), five flavones and flavone glycosides (14-18), two alkaloids (19, 20), one steroid (21), and one phenylpropanoid glycoside (22). The isolation and structural elucidation of the caffeoylated catechin (1) by means of spectroscopic techniques were described. We also provide the first evidence that 1 is synthesized via a two-step pathway in tea plant. The three HCCs (1-3) were investigated on their bioactivity through molecular modeling simulation and biochemical experiments. Our results show that they bind acetylcholinesterase (AChE) tightly and have strong AChE inhibitory activity with IC50 value at 2.49, 11.41, 62.26μM, respectively.Entities:
Keywords: (−)-Epicatechin (EC, PubChem CID: 72276); (−)-Epicatechin-3-O-gallate (ECG, PubChem CID: 107905); (−)-Epigallocatechin-3-O-gallate (EGCG, PubChem CID: 65064); Acetylcholinesterase inhibitory activity; Caffeic acid (PubChem CID: 689043); Caffeoyl-β-d-glucose (PubChem CID: 5281761); Camellia sinensis; Epigallocatechin 3-O-p-coumarate (PubChem CID: 6474788); Huperzine A (PubChem CID: 1253); Hydroxycinnamoylated catechins; Molecular modeling; UDP-glucose (UDP-G) (PubChem CID: 8629)
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Year: 2017 PMID： 28763966 DOI： 10.1016/j.foodchem.2017.06.011
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514