Valentina Bravatà1,2, Luigi Minafra1, Giusi Irma Forte1, Francesco Paolo Cammarata1, Giorgio Russo1, Federica Maria Di Maggio2, Giuseppa Augello3, Domenico Lio2, Maria Carla Gilardi1,4,5. 1. a Institute of Molecular Bioimaging and Physiology (IBFM)-CNR , Cefalù ( PA ), Italy. 2. b Department of Pathobiology and Medical Biotechnologies , University of Palermo , Palermo , Italy. 3. c Institute of Biomedicine and Molecular Immunology 'A. Monroy' (IBIM)-CNR , Palermo , Italy. 4. d Department of Health Sciences , Tecnomed Foundation, University of Milano-Bicocca , Milan , Italy. 5. e Nuclear Medicine , San Raffaele Scientific Institute , Milan , Italy.
Abstract
PURPOSE: Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. MATERIALS AND METHODS: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. RESULTS: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of 'senescence-associated secretory phenotype' (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. CONCLUSIONS: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.
PURPOSE:Ionizing radiation (IR) treatment activates inflammatory processes causing the release of a great amount of molecules able to affect the cell survival. The aim of this study was to analyze the cytokine signature of conditioned medium produced by non-tumorigenic mammary epithelial cell line MCF10A, as well as MCF7 and MDA-MB-231 breast cancer cell lines, after single high doses of IR in order to understand their role in high radiation response. MATERIALS AND METHODS: We performed a cytokine profile of irradiated conditioned media of MCF10A, MCF7 and MDA-MB-231 cell lines treated with 9 or 23 Gy, by Luminex and ELISA analyses. RESULTS: Overall, our results show that both 9 Gy and 23 Gy of IR induce the release within the first 72 h of cytokines and growth factors potentially able to influence the tumor outcome, with a dose-independent and cell-line dependent signature. Moreover, our results show that the cell-senescence phenomenon does not correlate with the amount of 'senescence-associated secretory phenotype' (SASP) molecules released in media. Thus, additional mechanisms are probably involved in this process. CONCLUSIONS: These data open the possibility to evaluate cytokine profile as useful marker in modulating the personalized radiotherapy in breast cancer care.
Entities:
Keywords:
Ionizing radiation; breast cancer; cytokines; inflammation
Authors: Valentina Bravatà; Luigi Minafra; Francesco Paolo Cammarata; Pietro Pisciotta; Debora Lamia; Valentina Marchese; Giada Petringa; Lorenzo Manti; Giuseppe Ap Cirrone; Maria Carla Gilardi; Giacomo Cuttone; Giusi Irma Forte; Giorgio Russo Journal: Br J Radiol Date: 2018-07-05 Impact factor: 3.039
Authors: Pascale Lejeune; Véronique Cruciani; Axel Berg-Larsen; Andreas Schlicker; Anne Mobergslien; Lisa Bartnitzky; Sandra Berndt; Sabine Zitzmann-Kolbe; Claudia Kamfenkel; Stefan Stargard; Stefanie Hammer; Jennifer S Jørgensen; Malene Jackerott; Carsten H Nielsen; Christoph A Schatz; Hartwig Hennekes; Jenny Karlsson; Alan S Cuthbertson; Dominik Mumberg; Urs B Hagemann Journal: J Immunother Cancer Date: 2021-10 Impact factor: 13.751
Authors: Valentina Bravatà; Claudia Cava; Luigi Minafra; Francesco Paolo Cammarata; Giorgio Russo; Maria Carla Gilardi; Isabella Castiglioni; Giusi Irma Forte Journal: Int J Mol Sci Date: 2018-04-04 Impact factor: 5.923