Literature DB >> 28762495

Preparation of Cell Cultures and Vaccinia Virus Stocks.

Catherine A Cotter1, Patricia L Earl1, Linda S Wyatt1, Bernard Moss1.   

Abstract

The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2017 by John Wiley & Sons, Inc.
Copyright © 2017 John Wiley & Sons, Inc.

Entities:  

Keywords:  cell culture; modified vaccinia virus Ankara; vaccinia virus

Mesh：

Year:  2017        PMID： 28762495     DOI： 10.1002/cpps.34

Source DB:  PubMed          Journal:  Curr Protoc Protein Sci        ISSN： 1934-3655


  7 in total

1.  Vaccinia Virus C9 Ankyrin Repeat/F-Box Protein Is a Newly Identified Antagonist of the Type I Interferon-Induced Antiviral State.

Authors:  Ruikang Liu; Bernard Moss
Journal:  J Virol       Date:  2018-04-13       Impact factor: 5.103

2.  Rapid and quantitative evaluation of vaccinia virus-induced host shutoff using newly generated cell lines stably expressing secreted Gaussia luciferase.

Authors:  Joshua A Molina; Zhilong Yang
Journal:  J Med Virol       Date:  2022-04-22       Impact factor: 20.693

3.  Generation of Recombinant Vaccinia Viruses.

Authors:  Linda S Wyatt; Patricia L Earl; Bernard Moss
Journal:  Curr Protoc Protein Sci       Date:  2017-08-01

4.  A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication.

Authors:  Shuai Cao; Joshua A Molina; Fernando Cantu; Candy Hernandez; Zhilong Yang
Journal:  mBio       Date:  2022-04-18       Impact factor: 7.786

5.  Asparagine Is a Critical Limiting Metabolite for Vaccinia Virus Protein Synthesis during Glutamine Deprivation.

Authors:  Anil Pant; Shuai Cao; Zhilong Yang
Journal:  J Virol       Date:  2019-06-14       Impact factor: 5.103

6.  Viral and metazoan poxins are cGAMP-specific nucleases that restrict cGAS-STING signalling.

Authors:  James B Eaglesham; Youdong Pan; Thomas S Kupper; Philip J Kranzusch
Journal:  Nature       Date:  2019-02-06       Impact factor: 69.504

7.  Poxvirus-encoded decapping enzymes promote selective translation of viral mRNAs.

Authors:  Fernando Cantu; Shuai Cao; Candy Hernandez; Pragyesh Dhungel; Joshua Spradlin; Zhilong Yang
Journal:  PLoS Pathog       Date:  2020-10-08       Impact factor: 6.823
  7 in total

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