Ivan Baccelli1, Gaétan Glauser2, Brigitte Mauch-Mani3. 1. Laboratory of Molecular and Cell Biology, Institute of Biology, University of Neuchâtel, Neuchâtel, 2000, Switzerland. 2. Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel, Neuchâtel, 2000, Switzerland. 3. Laboratory of Molecular and Cell Biology, Institute of Biology, University of Neuchâtel, Neuchâtel, 2000, Switzerland. Brigitte.Mauch@unine.ch.
Abstract
MAIN CONCLUSION: Endogenous levels of β-aminobutyric acid (BABA) increase after the molecular recognition of pathogen presence. BABA is accumulated differently during resistance or susceptibility to disease. The priming molecule β-aminobutyric acid has been recently shown to be a natural product of plants, and this has provided significance to the previous discovery of a perception mechanism in Arabidopsis. BABA levels were found to increase after abiotic stress or infection with virulent pathogens, but the role of endogenous BABA in defence has remained to be established. To investigate the biological significance of endogenous BABA variations during plant-pathogen interactions, we investigated how infections with virulent, avirulent (AvrRpt2), and non-pathogenic (hrpA) strains of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), as well as treatment with defence elicitors (Flg22 and AtPep2), affect the accumulation of BABA in Arabidopsis plants. We found that BABA levels increased more rapidly during resistance than susceptibility to Pst DC3000. In addition, BABA was accumulated during PAMP-triggered immunity (PTI) after infection with the non-pathogenic Pst DC3000 hrpA mutant, or treatment with elicitors. Importantly, treatment with Flg22 induced BABA rise in Columbia-0 plants but not in Wassilewskija-0 plants, which naturally possess a non-functional flagellin receptor. These results indicate that BABA levels are controlled by the plant's immune system, thus advancing the understanding of the biological role of plant produced BABA.
MAIN CONCLUSION: Endogenous levels of β-aminobutyric acid (BABA) increase after the molecular recognition of pathogen presence. BABA is accumulated differently during resistance or susceptibility to disease. The priming molecule β-aminobutyric acid has been recently shown to be a natural product of plants, and this has provided significance to the previous discovery of a perception mechanism in Arabidopsis. BABA levels were found to increase after abiotic stress or infection with virulent pathogens, but the role of endogenous BABA in defence has remained to be established. To investigate the biological significance of endogenous BABA variations during plant-pathogen interactions, we investigated how infections with virulent, avirulent (AvrRpt2), and non-pathogenic (hrpA) strains of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), as well as treatment with defence elicitors (Flg22 and AtPep2), affect the accumulation of BABA in Arabidopsis plants. We found that BABA levels increased more rapidly during resistance than susceptibility to Pst DC3000. In addition, BABA was accumulated during PAMP-triggered immunity (PTI) after infection with the non-pathogenic Pst DC3000 hrpA mutant, or treatment with elicitors. Importantly, treatment with Flg22 induced BABA rise in Columbia-0 plants but not in Wassilewskija-0 plants, which naturally possess a non-functional flagellin receptor. These results indicate that BABA levels are controlled by the plant's immune system, thus advancing the understanding of the biological role of plant produced BABA.
Authors: Estrella Luna; Marieke van Hulten; Yuhua Zhang; Oliver Berkowitz; Ana López; Pierre Pétriacq; Matthew A Sellwood; Beining Chen; Mike Burrell; Allison van de Meene; Corné M J Pieterse; Victor Flors; Jurriaan Ton Journal: Nat Chem Biol Date: 2014-04-28 Impact factor: 15.040
Authors: Zhiying Wang; Liangqiao Bian; Chenglin Mo; Hui Shen; Lan Juan Zhao; Kuan-Jui Su; Maciej Kukula; Jauh Tzuoh Lee; Daniel W Armstrong; Robert Recker; Joan Lappe; Lynda F Bonewald; Hong-Wen Deng; Marco Brotto Journal: Commun Biol Date: 2020-01-22