BACKGROUND: The use of mobile phones has been associated with an increased risk of developing certain type of cancer, especially in long term users. Therefore, this study was aimed to investigate the potential genotoxic effect of mobile phone radiofrequency exposure on human peripheral blood mononuclear cells in vitro. METHODS: The study population consisted in 14 healthy volunteers. After collection of two whole blood samples, the former was placed in a plastic rack, 1 cm from the chassis of a commercial mobile phone (900 MHz carrier frequency), which was activated by a 30-min call. The second blood sample was instead maintained far from mobile phones or other RF sources. The influence of mobile phone RF on DNA integrity was assessed by analyzing γ-H2AX foci in lymphocytes using immunofluorescence staining kit on AKLIDES. RESULTS: No measure of γ-H2AX foci was significantly influenced by mobile phone RF exposure, nor mobile phone exposure was associated with significant risk of genetic damages in vitro (odds ratio comprised between 0.27 and 1.00). CONCLUSIONS: The results of this experimental study demonstrate that exposure of human lymphocytes to a conventional 900 MHz RF emitted by a commercial mobile phone for 30 min does not significantly impact DNA integrity.
BACKGROUND: The use of mobile phones has been associated with an increased risk of developing certain type of cancer, especially in long term users. Therefore, this study was aimed to investigate the potential genotoxic effect of mobile phone radiofrequency exposure on human peripheral blood mononuclear cells in vitro. METHODS: The study population consisted in 14 healthy volunteers. After collection of two whole blood samples, the former was placed in a plastic rack, 1 cm from the chassis of a commercial mobile phone (900 MHz carrier frequency), which was activated by a 30-min call. The second blood sample was instead maintained far from mobile phones or other RF sources. The influence of mobile phone RF on DNA integrity was assessed by analyzing γ-H2AX foci in lymphocytes using immunofluorescence staining kit on AKLIDES. RESULTS: No measure of γ-H2AX foci was significantly influenced by mobile phone RF exposure, nor mobile phone exposure was associated with significant risk of genetic damages in vitro (odds ratio comprised between 0.27 and 1.00). CONCLUSIONS: The results of this experimental study demonstrate that exposure of human lymphocytes to a conventional 900 MHz RF emitted by a commercial mobile phone for 30 min does not significantly impact DNA integrity.
Entities:
Keywords:
DNA damage; Mobile phone; cancer; lymphocytes; radiofrequency; γ-H2AX foci
Authors: L Verschaeve; J Juutilainen; I Lagroye; J Miyakoshi; R Saunders; R de Seze; T Tenforde; E van Rongen; B Veyret; Z Xu Journal: Mutat Res Date: 2010-10-16 Impact factor: 2.433
Authors: Alesia N Ivashkevich; Olga A Martin; Andrea J Smith; Christophe E Redon; William M Bonner; Roger F Martin; Pavel N Lobachevsky Journal: Mutat Res Date: 2011-01-07 Impact factor: 2.433
Authors: L Verschaeve; P Heikkinen; G Verheyen; U Van Gorp; F Boonen; F Vander Plaetse; A Maes; T Kumlin; J Mäki-Paakkanen; L Puranen; J Juutilainen Journal: Radiat Res Date: 2006-05 Impact factor: 2.841
Authors: Matus Durdik; Pavol Kosik; Eva Markova; Alexandra Somsedikova; Beata Gajdosechova; Ekaterina Nikitina; Eva Horvathova; Katarina Kozics; Devra Davis; Igor Belyaev Journal: Sci Rep Date: 2019-11-07 Impact factor: 4.379