Literature DB >> 2875768

A new method for producing a specific and high titre antibody against glutamate using colloidal gold as a carrier.

S Shiosaka, H Kiyama, A Wanaka, M Tohyama.   

Abstract

Antiserum against the glutamate was developed using colloidal gold as a carrier for the antigen. Glutamate was bound directly to colloidal gold without using cross-linking reagents such as glutaraldehyde, formaldehyde, or carbodiimide. Repeated injection of the colloidal gold-glutamate complex into the rabbit yielded a specific, high titre antiserum. Specificity was verified by radioimmunoassay and histochemically with absorption controls. The antiserum immunohistochemically detected numerous glutamate-containing neurons in the rat brain.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 2875768     DOI： 10.1016/0006-8993(86)91352-1

Source DB:  PubMed          Journal:  Brain Res        ISSN： 0006-8993            Impact factor:   3.252


  11 in total

Review 1.  Quantitative electron microscopic immunocytochemistry of neuroactive amino acids.

Authors:  O P Ottersen
Journal:  Anat Embryol (Berl)       Date:  1989

2.  Glutamate-like immunoreactivity revealed in rat olfactory bulb, hippocampus and cerebellum by monoclonal antibody and sensitive staining method.

Authors:  C J Liu; P Grandes; C Matute; M Cuénod; P Streit
Journal:  Histochemistry       Date:  1989

3.  Obtaining and Characteristic of Antibodies to Vibrio cholerae Protective Antigens Conjugated with Gold Nanoparticles.

Authors:  L A Dykman; O A Volokh; O V Gromova; O S Durakova; S A Vorobeva; M N Kireev; L F Livanova; A K Nikiforov; S Y Shchyogolev; V V Kutyrev
Journal:  Dokl Biochem Biophys       Date:  2020-04-27       Impact factor: 0.788

4.  Glutamate-like immunoreactive structures in primary sensory neurons in the rat detected by a specific antiserum against glutamate.

Authors:  A Wanaka; Y Shiotani; H Kiyama; T Matsuyama; T Kamada; S Shiosaka; M Tohyama
Journal:  Exp Brain Res       Date:  1987       Impact factor: 1.972

5.  Antibodies to quinolinic acid and the determination of its cellular distribution within the rat immune system.

Authors:  J R Moffett; M G Espey; M A Namboodiri
Journal:  Cell Tissue Res       Date:  1994-12       Impact factor: 5.249

Review 6.  Immunomodulatory Nanosystems.

Authors:  Xiangru Feng; Weiguo Xu; Zhongmin Li; Wantong Song; Jianxun Ding; Xuesi Chen
Journal:  Adv Sci (Weinh)       Date:  2019-06-21       Impact factor: 16.806

7.  Gold nanoparticles in biology and medicine: recent advances and prospects.

Authors:  L A Dykman; N G Khlebtsov
Journal:  Acta Naturae       Date:  2011-04       Impact factor: 1.845

8.  Immunological properties of gold nanoparticles.

Authors:  Lev A Dykman; Nikolai G Khlebtsov
Journal:  Chem Sci       Date:  2016-11-16       Impact factor: 9.825

9.  Preparation and immunological properties of a nanovaccine against Pseudomonas aeruginosa based on gold nanoparticles and detoxified lipopolysaccharide.

Authors:  Faezeh Najafzadeh; Asghar Tanomand; Azam Haddadi; Jafar Majidi
Journal:  Iran J Basic Med Sci       Date:  2021-02       Impact factor: 2.699

Review 10.  Gold nanoparticles for preparation of antibodies and vaccines against infectious diseases.

Authors:  Lev A Dykman
Journal:  Expert Rev Vaccines       Date:  2020-04-28       Impact factor: 5.217
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.