AIM: The aim of this study is to examine the elevation of MYOC in long-term treatment of human trabecular meshwork (HTM) cells using dexamethasone (DEX) encapsulated pentablock (PB) copolymer-based nanoparticles (NPs) (DEX-PB-NPs). MATERIALS & METHODS: PB copolymers and DEX-PB-NPs were synthesized and characterized using nuclear magnetic resonance, gel permeation chromatography, and X-ray diffraction analyses. MYOC levels secreted from HTM cells were measured by western blot (WB) analysis. RESULTS: DEX-PB-NPs were formulated in the size range of 109 ± 3.77 nm (n = 3). A long term DEX release from the NPs was observed over three months. Cell viability and cytotoxicity were not affected up to 12 weeks of treatment with PB-copolymer or DEX-PB-NPs. WB data from five HTM cell strains showed that MYOC levels increased by 5.2 ± 1.3, 7.4 ± 4.3, and 2.8 ± 1.1-fold in the presence of DEX-PB-NPs compared with 9.2 ± 3.8, 2.2 ± 0.5, and 1.5 ± 0.3-fold at 4, 8 and 12 weeks in control-DEX treatment group, respectively (n = 5). Based on the decline in MYOC levels after withdrawal of DEX from control wells, DEX-PB-NPs released the DEX for at least 10 weeks. CONCLUSION: The treatment of HTM cells using DEX-PB-NPs were analyzed in this study. The in vitro cell-based system developed here is a valuable tool for determining the safety and effects of steroids released from polymeric NPs.
AIM: The aim of this study is to examine the elevation of MYOC in long-term treatment of human trabecular meshwork (HTM) cells using dexamethasone (DEX) encapsulated pentablock (PB) copolymer-based nanoparticles (NPs) (DEX-PB-NPs). MATERIALS & METHODS:PB copolymers and DEX-PB-NPs were synthesized and characterized using nuclear magnetic resonance, gel permeation chromatography, and X-ray diffraction analyses. MYOC levels secreted from HTM cells were measured by western blot (WB) analysis. RESULTS:DEX-PB-NPs were formulated in the size range of 109 ± 3.77 nm (n = 3). A long term DEX release from the NPs was observed over three months. Cell viability and cytotoxicity were not affected up to 12 weeks of treatment with PB-copolymer or DEX-PB-NPs. WB data from five HTM cell strains showed that MYOC levels increased by 5.2 ± 1.3, 7.4 ± 4.3, and 2.8 ± 1.1-fold in the presence of DEX-PB-NPs compared with 9.2 ± 3.8, 2.2 ± 0.5, and 1.5 ± 0.3-fold at 4, 8 and 12 weeks in control-DEX treatment group, respectively (n = 5). Based on the decline in MYOC levels after withdrawal of DEX from control wells, DEX-PB-NPs released the DEX for at least 10 weeks. CONCLUSION: The treatment of HTM cells using DEX-PB-NPs were analyzed in this study. The in vitro cell-based system developed here is a valuable tool for determining the safety and effects of steroids released from polymeric NPs.
Entities:
Keywords:
MYOC; block copolymer; nanoparticle; steroids; sustained release; trabecular meshwork cells; western blot
Authors: Julia A Haller; Francesco Bandello; Rubens Belfort; Mark S Blumenkranz; Mark Gillies; Jeffrey Heier; Anat Loewenstein; Young Hee Yoon; Jenny Jiao; Xiao-Yan Li; Scott M Whitcup; Joanne Li Journal: Ophthalmology Date: 2011-07-20 Impact factor: 12.079
Authors: Sai H S Boddu; Jwala Jwala; Ravi Vaishya; Ravinder Earla; Pradeep K Karla; Dhananjay Pal; Ashim K Mitra Journal: J Ocul Pharmacol Ther Date: 2010-02 Impact factor: 2.671
Authors: Christian Gerecke; Alexander Edlich; Michael Giulbudagian; Fabian Schumacher; Nan Zhang; Andre Said; Guy Yealland; Silke B Lohan; Falko Neumann; Martina C Meinke; Nan Ma; Marcelo Calderón; Sarah Hedtrich; Monika Schäfer-Korting; Burkhard Kleuser Journal: Nanotoxicology Date: 2017-02-22 Impact factor: 5.913
Authors: Jacques A Bertrand; David F Woodward; Joseph M Sherwood; Jenny W Wang; Darryl R Overby Journal: Br J Ophthalmol Date: 2020-11-25 Impact factor: 4.638
Authors: Guorong Li; Chanyoung Lee; A Thomas Read; Ke Wang; Jungmin Ha; Megan Kuhn; Iris Navarro; Jenny Cui; Katherine Young; Rahul Gorijavolu; Todd Sulchek; Casey Kopczynski; Sina Farsiu; John Samples; Pratap Challa; C Ross Ethier; W Daniel Stamer Journal: Elife Date: 2021-03-30 Impact factor: 8.140
Authors: Ke Wang; Guorong Li; A Thomas Read; Iris Navarro; Ashim K Mitra; W Daniel Stamer; Todd Sulchek; C Ross Ethier Journal: Sci Rep Date: 2018-04-11 Impact factor: 4.379