| Literature DB >> 28756340 |
Alexander Plotnikov1, Noga Kozer1, Vladislav Krupalnik2, Shani Peles2, Nofar Mor2, Yoach Rais2, Jacob H Hanna2, Haim M Barr3.
Abstract
Measurement of Alkaline Phosphatase (ALP) level is a widely used procedure in clinical and basic research. We present a simple and inexpensive luminescence-based method that allows multiplexed measurement and normalization of intracellular ALP levels in one sample well. The method comprises two commercially available reagents enabling quantification of ALP levels and cell number by two sequential luminescence readouts. Using this method we were able to detect and analyze somatic reprogramming into pluripotent stem cells. The method is highly applicable for High Throughput Screening (HTS) campaigns and analysis.Entities:
Keywords: ATP; Alkaline; Differentiation; Drug; Phosphatase; Screening
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Year: 2017 PMID： 28756340 DOI： 10.1016/j.scr.2017.07.014
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020