| Literature DB >> 28755442 |
Paula Teper-Bamnolker1, Yossi Buskila1,2, Eduard Belausov3, Dalia Wolf4, Adi Doron-Faigenboim5, Shifra Ben-Dor6, Renier A L Van der Hoorn7, Amnon Lers1, Dani Eshel1.
Abstract
The potato (Solanum tuberosum L.) tuber is a swollen underground stem that can sprout in an apical dominance (AD) pattern. Bromoethane (BE) induces loss of AD and the accumulation of vegetative vacuolar processing enzyme (S. tuberosum vacuolar processing enzyme [StVPE]) in the tuber apical meristem (TAM). Vacuolar processing enzyme activity, induced by BE, is followed by programmed cell death in the TAM. In this study, we found that the mature StVPE1 (mVPE) protein exhibits specific activity for caspase 1, but not caspase 3 substrates. Optimal activity of mVPE was achieved at acidic pH, consistent with localization of StVPE1 to the vacuole, at the edge of the TAM. Downregulation of StVPE1 by RNA interference resulted in reduced stem branching and retained AD in tubers treated with BE. Overexpression of StVPE1 fused to green fluorescent protein showed enhanced stem branching after BE treatment. Our data suggest that, following stress, induction of StVPE1 in the TAM induces AD loss and stem branching.Entities:
Keywords: Solanum tuberosum (potato); apical dominance (AD); caspase; stem branching; tuber apical meristem (TAM); vacuolar processing enzyme (VPE)
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Year: 2017 PMID： 28755442 DOI： 10.1111/pce.13044
Source DB: PubMed Journal: Plant Cell Environ ISSN： 0140-7791 Impact factor: 7.228