F Lesage1, S Pranpanus2, F M Bosisio3,4, M Jacobs1, S Ospitalieri1, J Toelen1,5, J Deprest6,7. 1. Department of Development and Regeneration, KU Leuven-University of Leuven, Leuven, Belgium. 2. Department of Obstetrics and Gynecology, Prince of Songkla University, Songkhla, Thailand. 3. Department of Imaging and Pathology, KU Leuven-University of Leuven, Leuven, Belgium. 4. Università Degli Studi di Milano-Bicocca, Milan, Italy. 5. Department of Pediatrics, University Hospitals Leuven, Leuven, Belgium. 6. Department of Development and Regeneration, KU Leuven-University of Leuven, Leuven, Belgium. jan.deprest@uzleuven.be. 7. Department of Obstetrics and Gynecology, University Hospitals Leuven, Herestraat 49, 3000, Leuven, Belgium. jan.deprest@uzleuven.be.
Abstract
PURPOSE: Surgical restoration of soft tissue defects often requires implantable devices. The clinical outcome of the surgery is determined by the properties inherent to the used matrix. Mesenchymal stem cells (MSC) modulate the immune processes after in vivo transplantation and their addition to matrices is associated with constructive remodeling. Herein we evaluate the potential of MSC derived from the amniotic fluid (AF-MSC), an interesting MSC source for cell therapeutic applications in the perinatal period, for immune modulation when added to a biomaterial. METHODS: We implant cell free small intestinal submucosa (SIS) or SIS seeded with AF-MSC at a density of 1 × 105/cm2 subcutaneously at the abdominal wall in immune competent rats. The host immune response is evaluated at 3, 7 and 14 days postoperatively. RESULTS: The matrix-specific or cellular characteristics are not altered after 24 h of in vitro co-culture of SIS with AF-MSC. The host immune response was not different between animals implanted with cell free or AF-MSC-seeded SIS in terms of cellular infiltration, vascularity, macrophage polarization or scaffold replacement. Profiling the mRNA expression level of inflammatory cytokines at the matrix interface shows a significant reduction in the expression of the pro-inflammatory marker Tnf-α and a trend towards lower iNos expression upon AF-MSC-seeding of the SIS matrix. Anti-inflammatory marker expression does not alter upon cell seeding of matrix implants. CONCLUSION: We conclude that SIS is a suitable substrate for in vitro culture of AF-MSC and fibroblasts. AF-MSC addition to SIS does not significantly modulate the host immune response after subcutaneous implantation in rats.
PURPOSE: Surgical restoration of soft tissue defects often requires implantable devices. The clinical outcome of the surgery is determined by the properties inherent to the used matrix. Mesenchymal stem cells (MSC) modulate the immune processes after in vivo transplantation and their addition to matrices is associated with constructive remodeling. Herein we evaluate the potential of MSC derived from the amniotic fluid (AF-MSC), an interesting MSC source for cell therapeutic applications in the perinatal period, for immune modulation when added to a biomaterial. METHODS: We implant cell free small intestinal submucosa (SIS) or SIS seeded with AF-MSC at a density of 1 × 105/cm2 subcutaneously at the abdominal wall in immune competent rats. The host immune response is evaluated at 3, 7 and 14 days postoperatively. RESULTS: The matrix-specific or cellular characteristics are not altered after 24 h of in vitro co-culture of SIS with AF-MSC. The host immune response was not different between animals implanted with cell free or AF-MSC-seeded SIS in terms of cellular infiltration, vascularity, macrophage polarization or scaffold replacement. Profiling the mRNA expression level of inflammatory cytokines at the matrix interface shows a significant reduction in the expression of the pro-inflammatory marker Tnf-α and a trend towards lower iNos expression upon AF-MSC-seeding of the SIS matrix. Anti-inflammatory marker expression does not alter upon cell seeding of matrix implants. CONCLUSION: We conclude that SIS is a suitable substrate for in vitro culture of AF-MSC and fibroblasts. AF-MSC addition to SIS does not significantly modulate the host immune response after subcutaneous implantation in rats.
Authors: A J Allman; T B McPherson; S F Badylak; L C Merrill; B Kallakury; C Sheehan; R H Raeder; D W Metzger Journal: Transplantation Date: 2001-06-15 Impact factor: 4.939
Authors: Stephen F Badylak; Jolene E Valentin; Anjani K Ravindra; George P McCabe; Ann M Stewart-Akers Journal: Tissue Eng Part A Date: 2008-11 Impact factor: 3.845
Authors: Maja L Konstantinovic; Pieter Lagae; Fang Zheng; Eric K Verbeken; Dirk De Ridder; Jan A Deprest Journal: BJOG Date: 2005-11 Impact factor: 6.531
Authors: Marina Gabriela Monteiro Carvalho Mori da Cunha; Silvia Zia; Fanny Oliveira Arcolino; Marianne Sylvia Carlon; Diego Vilibaldo Beckmann; Ney Luis Pippi; Dominguita Luhers Graça; Elena Levtchenko; Jan Deprest; Jaan Toelen Journal: PLoS One Date: 2015-08-21 Impact factor: 3.240