| Literature DB >> 28752292 |
So-Young Lee1, Hee-Youn Kim1, Byoung-Jun Kim1, Hong Kim1, Seung-Hyeok Seok1, Bum-Joon Kim1,2,3, Yoon-Hoh Kook4.
Abstract
Cultivation of the smooth colony Mycobacterium abscessus at the sub-minimum inhibitory concentration (MIC) of amikacin changed its growth pattern including its colony morphology (smooth to rough) and cell arrangement (dispersed to cord formation). In addition, reduced sliding motility and biofilm formation were observed. The amount of glycogpetidolipid (GPL) and mRNA expression of key genes involved in GPL synthesis were decreased in the amikacin-treated M. abscessus strain. An in vitro infection assay revealed that the amikacin-treated smooth M. abscessus strain induced more pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) than that of the smooth strain in murine macrophage cells. These results suggest that long-term exposure to a low concentration of amikacin causes a physical change in the cell wall which may increase its virulence.Entities:
Keywords: Mycobacterium abscessus; amikacin; biofilm; glycopeptidolipid (GPL)
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Year: 2017 PMID： 28752292 DOI： 10.1007/s12275-017-6503-7
Source DB: PubMed Journal: J Microbiol ISSN： 1225-8873 Impact factor: 3.422