Ester Martínez-Sarrà1,2, Sheyla Montori1, Carlos Gil-Recio1, Raquel Núñez-Toldrà1, Domiziana Costamagna2, Alessio Rotini2,3,4, Maher Atari1, Aernout Luttun5, Maurilio Sampaolesi6,7. 1. Regenerative Medicine Research Institute, Universitat Internacional de Catalunya, Barcelona, 08017, Spain. 2. Translational Cardiomyology Laboratory, Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, Leuven, 3000, Belgium. 3. Department of Neuroscience, Imaging and Clinical Sciences, University "G. d'Annunzio", Chieti, 66100, Italy. 4. Interuniversity Institute of Myology, Chieti, 66100, Italy. 5. Centre for Molecular and Vascular Biology, Department of Cardiovascular Sciences, KU Leuven, Leuven, 3000, Belgium. 6. Translational Cardiomyology Laboratory, Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, Leuven, 3000, Belgium. Maurilio.Sampaolesi@kuleuven.be. 7. Human Anatomy Unit, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, 27100, Italy. Maurilio.Sampaolesi@kuleuven.be.
Abstract
BACKGROUND: Dental pulp represents an easily accessible autologous source of adult stem cells. A subset of these cells, named dental pulp pluripotent-like stem cells (DPPSC), shows high plasticity and can undergo multiple population doublings, making DPPSC an appealing tool for tissue repair or maintenance. METHODS: DPPSC were harvested from the dental pulp of third molars extracted from young patients. Growth factors released by DPPSC were analysed using antibody arrays. Cells were cultured in specific differentiation media and their endothelial, smooth and skeletal muscle differentiation potential was evaluated. The therapeutic potential of DPPSC was tested in a wound healing mouse model and in two genetic mouse models of muscular dystrophy (Scid/mdx and Sgcb-null Rag2-null γc-null). RESULTS: DPPSC secreted several growth factors involved in angiogenesis and extracellular matrix deposition and improved vascularisation in all three murine models. Moreover, DPPSC stimulated re-epithelialisation and ameliorated collagen deposition and organisation in healing wounds. In dystrophic mice, DPPSC engrafted in the skeletal muscle of both dystrophic murine models and showed integration in muscular fibres and vessels. In addition, DPPSC treatment resulted in reduced fibrosis and collagen content, larger cross-sectional area of type II fast-glycolytic fibres and infiltration of higher numbers of proangiogenic CD206+ macrophages. CONCLUSIONS: Overall, DPPSC represent a potential source of stem cells to enhance the wound healing process and slow down dystrophic muscle degeneration.
BACKGROUND: Dental pulp represents an easily accessible autologous source of adult stem cells. A subset of these cells, named dental pulp pluripotent-like stem cells (DPPSC), shows high plasticity and can undergo multiple population doublings, making DPPSC an appealing tool for tissue repair or maintenance. METHODS:DPPSC were harvested from the dental pulp of third molars extracted from young patients. Growth factors released by DPPSC were analysed using antibody arrays. Cells were cultured in specific differentiation media and their endothelial, smooth and skeletal muscle differentiation potential was evaluated. The therapeutic potential of DPPSC was tested in a wound healing mouse model and in two genetic mouse models of muscular dystrophy (Scid/mdx and Sgcb-null Rag2-null γc-null). RESULTS:DPPSC secreted several growth factors involved in angiogenesis and extracellular matrix deposition and improved vascularisation in all three murine models. Moreover, DPPSC stimulated re-epithelialisation and ameliorated collagen deposition and organisation in healing wounds. In dystrophicmice, DPPSC engrafted in the skeletal muscle of both dystrophicmurine models and showed integration in muscular fibres and vessels. In addition, DPPSC treatment resulted in reduced fibrosis and collagen content, larger cross-sectional area of type II fast-glycolytic fibres and infiltration of higher numbers of proangiogenic CD206+ macrophages. CONCLUSIONS: Overall, DPPSC represent a potential source of stem cells to enhance the wound healing process and slow down dystrophic muscle degeneration.
Authors: Luc Sensebé; Karin Tarte; Jacques Galipeau; Mauro Krampera; Ivan Martin; Donald G Phinney; Yufang Shi Journal: Cell Stem Cell Date: 2012-01-06 Impact factor: 24.633
Authors: M Rius; A Obradors; G Daina; J Cuzzi; L Marquès; G Calderón; E Velilla; O Martínez-Passarell; M Oliver-Bonet; J Benet; J Navarro Journal: Hum Reprod Date: 2010-05-19 Impact factor: 6.918
Authors: Maher Atari; Carlos Gil-Recio; Marc Fabregat; Dani García-Fernández; Miguel Barajas; Miguel A Carrasco; Han-Sung Jung; F Hernández Alfaro; Nuria Casals; Felipe Prosper; Eduard Ferrés-Padró; Luis Giner Journal: J Cell Sci Date: 2012-03-30 Impact factor: 5.285
Authors: Annelies Bronckaers; Petra Hilkens; Yanick Fanton; Tom Struys; Pascal Gervois; Constantinus Politis; Wendy Martens; Ivo Lambrichts Journal: PLoS One Date: 2013-08-07 Impact factor: 3.240