Tracy McMillen1, Yeon Joo Lee2, Mini Kamboj3, N Esther Babady4. 1. Clinical Microbiology Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, United States. 2. Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, United States. 3. Department of Laboratory Medicine and Infection Control Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, United States; Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, United States. 4. Clinical Microbiology Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, United States; Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, United States. Electronic address: babadyn@mskcc.org.
Abstract
BACKGROUND: Diagnosis of Adenovirus infections in transplant patients may be accomplished using either plasma or stool samples. IVD-cleared multiplexed gastrointestinal (GI) PCR panels offer an option for rapid testing of stool samples but most only target Adenovirus (HAdV) types F40/41. OBJECTIVES: Given the potential significance of a positive adenovirus test in an immunocompromised patient, we sought to determine the frequency of type 40/41 in our patient population and the utility of a readily available multiplexed, FDA-cleared GI Panel for the detection of adenovirus infections. STUDY DESIGN: A total of 215 specimens from immunocompromised patients mostly with hematologic malignancy or transplant recipients were evaluated including 107 plasma samples, 85 stool samples and 23 respiratory samples. Genotyping was performed successfully on 122 specimens. RESULTS: The most common type detected in all samples including stools was Adenovirus C/2. In a subset of patients with multiple specimen types tested, similar types were detected in all samples. CONCLUSIONS: Although Adenovirus F40/41 is the most common enteric type, Adenovirus C/2 was the most common type identified in stools and subsequently plasma samples of our patient population. Implementation of assays that have wide reactivity for most adenovirus types is essential for optimal diagnostic yield.
BACKGROUND: Diagnosis of Adenovirus infections in transplant patients may be accomplished using either plasma or stool samples. IVD-cleared multiplexed gastrointestinal (GI) PCR panels offer an option for rapid testing of stool samples but most only target Adenovirus (HAdV) types F40/41. OBJECTIVES: Given the potential significance of a positive adenovirus test in an immunocompromised patient, we sought to determine the frequency of type 40/41 in our patient population and the utility of a readily available multiplexed, FDA-cleared GI Panel for the detection of adenovirus infections. STUDY DESIGN: A total of 215 specimens from immunocompromised patients mostly with hematologic malignancy or transplant recipients were evaluated including 107 plasma samples, 85 stool samples and 23 respiratory samples. Genotyping was performed successfully on 122 specimens. RESULTS: The most common type detected in all samples including stools was Adenovirus C/2. In a subset of patients with multiple specimen types tested, similar types were detected in all samples. CONCLUSIONS: Although Adenovirus F40/41 is the most common enteric type, Adenovirus C/2 was the most common type identified in stools and subsequently plasma samples of our patient population. Implementation of assays that have wide reactivity for most adenovirus types is essential for optimal diagnostic yield.
Authors: Maria Antonia De Francesco; Giovanni Lorenzin; Antonella Meini; Richard Fabian Schumacher; Arnaldo Caruso Journal: Microbiol Spectr Date: 2021-07-28