Literature DB >> 2874903

Improved assay of the enzymes of glutathione synthesis: gamma-glutamylcysteine synthetase and glutathione synthetase.

E Beutler, T Gelbart.   

Abstract

New methods for the estimation of red cell gamma-glutamylcysteine synthetase and glutathione synthetase have been developed. gamma-32P ATP is allowed to equilibrate until the gamma and beta phosphate groups are equally labelled. The amount of 32Pi released in the presence of glutamic acid and cysteine, the substrates for GC-S or in the presence of gamma-glutamylcysteine and glycine, the substrates of GSH-S, is measured. This is accomplished by extraction of the phosphomolybdate complex into isobutanol-benzene. The methods are linear with time and hemolysate concentration. Normal values are presented.

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Year:  1986        PMID： 2874903     DOI： 10.1016/0009-8981(86)90122-1

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  6 in total

1.  Inhibition of Radiation-Induced Oxidative Damage in the Lung Tissue: May Acetylsalicylic Acid Have a Positive Role?

Authors:  Can Demirel; Sevil Cagiran Kilciksiz; Serkan Gurgul; Nurten Erdal; Seyran Yigit; Lulufer Tamer; Lokman Ayaz
Journal:  Inflammation       Date:  2016-02       Impact factor: 4.092

2.  Characterization and immunological properties of selenium-containing glutathione peroxidase induced by selenite in Chlamydomonas reinhardtii.

Authors:  S Shigeoka; T Takeda; T Hanaoka
Journal:  Biochem J       Date:  1991-05-01       Impact factor: 3.857

3.  Protective role of glutathione synthesis on radiation-induced DNA damage in rabbit brain.

Authors:  T Shimizu; M Iwanaga; A Yasunaga; Y Urata; S Goto; S Shibata; T Kondo
Journal:  Cell Mol Neurobiol       Date:  1998-06       Impact factor: 5.046

4.  Weakened cellular scavenging activity against oxidative stress in diabetes mellitus: regulation of glutathione synthesis and efflux.

Authors:  K Yoshida; J Hirokawa; S Tagami; Y Kawakami; Y Urata; T Kondo
Journal:  Diabetologia       Date:  1995-02       Impact factor: 10.122

5.  Neuroprotective role of vitamin D in primary neuronal cortical culture.

Authors:  Reham AlJohri; Majid AlOkail; Samina Hyder Haq
Journal:  eNeurologicalSci       Date:  2018-12-17

6.  Therapeutic Potential of Vitamin D and Curcumin in an In Vitro Model of Alzheimer Disease.

Authors:  Abir Abdullah Alamro; Ebtesam Atiah Alsulami; Moudhi Almutlaq; Amani Alghamedi; Majed Alokail; Samina Hyder Haq
Journal:  J Cent Nerv Syst Dis       Date:  2020-05-27
  6 in total

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