D E Agbonlahor1, A Erah2, I M Agba3, F E Oviasogie4, A F Ehiaghe5, M Wankasi6, O A Eremwanarue7, I J Ehiaghe7, E C Ogbu7, R I Iyen7, S Abbey6, M Y Tatfeng6, J Uhunmwangho4. 1. Lahor Research Laboratories and Medical Centre, Benin City, Edo State; Department of Medical Laboratory Science, College of Health Sciences, Niger Delta University, Amassoma, Bayelsa State, Nigeria. 2. Department of Medical Laboratory Science, Igbinedion University, Okada, Nigeria. 3. Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria. 4. Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria. 5. Lahor Research Laboratories and Medical Centre, Benin City, Edo State; Department of Medical Laboratory Science, Igbinedion University, Okada, Nigeria. 6. Department of Medical Laboratory Science, College of Health Sciences, Niger Delta University, Amassoma, Bayelsa State, Nigeria. 7. Lahor Research Laboratories and Medical Centre, Benin City, Edo State, Nigeria.
Abstract
BACKGROUND & OBJECTIVES: Lassa fever has been endemic in Nigeria since 1969. The rodent Mastomys natalensis has been widely claimed to be the reservoir host of the Lassa virus. This study was designed to investigate the dis- tribution of species of rodents in three states (Edo, Delta and Bayelsa) of Nigeria and to determine the prevalence of Lassa virus amongst trapped rodents in the selected states. METHODS: Rodents were trapped during November 2015 to October 2016 from the three states in South-South re- gion of Nigeria. Total RNA was extracted from the blood collected from the trapped rodents. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm the presence of Lassa virus in the rodents. RESULTS: The results revealed that six species of rodents were predominantly present in these geographical locations. Mus musculus (39.4%) had the highest prevalence, closely followed by Rattus rattus (36.1%), R. fuscipus (20.3%), M. natalensis (2%), Myosoricinae soricidae (1.2%) and R. norvegicus (1%). The overall positivity (carrier rate) of Lassa virus was 1.6% amongst the 1500 rodents caught in the three states. In Edo and Delta States, the RT-PCR results showed presence of Lassa virus in R. rattus, M. musculus and M. natalensis. On the other hand, only M. na- talensis was detected with the virus, amongst the species of rodents caught in Bayelsa State. M. natalensis recorded the highest Lassa virus among rodents trapped in Edo (87%), Delta (50%) and Bayelsa (11%) States respectively. INTERPRETATION & CONCLUSION: The rather low Lassa virus positive among rodents in Bayelsa State of Nigeria may explain the absence of reports of outbreak of Lassa fever over the past 48 yr in the state. The results also confirmed that apart from Mastomys natalensis, other rodents such as Rattus rattus and Mus musculus may also serve as res- ervoirs for Lassa virus. From the findings of this cross-sectional study, it was concluded that a more comprehensive study on rodents as reservoir host, need to be undertaken across the entire states of Nigeria, for better understanding of the epidemiology and endemicity of Lassa fever.
BACKGROUND & OBJECTIVES: Lassa fever has been endemic in Nigeria since 1969. The rodent Mastomys natalensis has been widely claimed to be the reservoir host of the Lassa virus. This study was designed to investigate the dis- tribution of species of rodents in three states (Edo, Delta and Bayelsa) of Nigeria and to determine the prevalence of Lassa virus amongst trapped rodents in the selected states. METHODS: Rodents were trapped during November 2015 to October 2016 from the three states in South-South re- gion of Nigeria. Total RNA was extracted from the blood collected from the trapped rodents. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm the presence of Lassa virus in the rodents. RESULTS: The results revealed that six species of rodents were predominantly present in these geographical locations. Mus musculus (39.4%) had the highest prevalence, closely followed by Rattus rattus (36.1%), R. fuscipus (20.3%), M. natalensis (2%), Myosoricinae soricidae (1.2%) and R. norvegicus (1%). The overall positivity (carrier rate) of Lassa virus was 1.6% amongst the 1500 rodents caught in the three states. In Edo and Delta States, the RT-PCR results showed presence of Lassa virus in R. rattus, M. musculus and M. natalensis. On the other hand, only M. na- talensis was detected with the virus, amongst the species of rodents caught in Bayelsa State. M. natalensis recorded the highest Lassa virus among rodents trapped in Edo (87%), Delta (50%) and Bayelsa (11%) States respectively. INTERPRETATION & CONCLUSION: The rather low Lassa virus positive among rodents in Bayelsa State of Nigeria may explain the absence of reports of outbreak of Lassa fever over the past 48 yr in the state. The results also confirmed that apart from Mastomys natalensis, other rodents such as Rattus rattus and Mus musculus may also serve as res- ervoirs for Lassa virus. From the findings of this cross-sectional study, it was concluded that a more comprehensive study on rodents as reservoir host, need to be undertaken across the entire states of Nigeria, for better understanding of the epidemiology and endemicity of Lassa fever.
Authors: Anise N Happi; Testimony J Olumade; Olusola A Ogunsanya; Ayotunde E Sijuwola; Seto C Ogunleye; Judith U Oguzie; Cecilia Nwofoke; Chinedu A Ugwu; Samuel J Okoro; Patricia I Otuh; Louis N Ngele; Oluwafemi O Ojo; Ademola Adelabu; Roseline F Adeleye; Nicholas E Oyejide; Clinton S Njaka; Jonathan L Heeney; Christian T Happi Journal: Microbiol Spectr Date: 2022-08-01