Literature DB >> 28748203

Thermal Stability of Heterotrimeric pMHC Proteins as Determined by Circular Dichroism Spectroscopy.

Anna Fuller1, Aaron Wall1, Michael D Crowther1, Angharad Lloyd1, Alexei Zhurov2, Andrew K Sewell1, David K Cole1, Konrad Beck2.   

Abstract

T cell receptor (TCR) recognition of foreign peptide fragments, presented by peptide major histocompatibility complex (pMHC), governs T-cell mediated protection against pathogens and cancer. Many factors govern T-cell sensitivity, including the affinity of the TCR-pMHC interaction and the stability of pMHC on the surface of antigen presenting cells. These factors are particularly relevant for the peptide vaccination field, in which more stable pMHC interactions could enable more effective protection against disease. Here, we discuss a method for the determination of pMHC stability that we have used to investigate HIV immune escape, T-cell sensitivity to cancer antigens and mechanisms leading to autoimmunity.

Entities:  

Keywords:  Circular dichroism; Peptide vaccines; Peptide-MHC stability; Protein folding; Recombinant protein; T-cells; Thermal stability

Year:  2017        PMID： 28748203      PMCID： PMC5524174          DOI： 10.21769/BioProtoc.2366

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  19 in total

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Journal:  J Immunol       Date:  1992-12-01       Impact factor: 5.422

2.  Calculating thermodynamic data for transitions of any molecularity from equilibrium melting curves.

Authors:  L A Marky; K J Breslauer
Journal:  Biopolymers       Date:  1987-09       Impact factor: 2.505

3.  Structure of a TCR with high affinity for self-antigen reveals basis for escape from negative selection.

Authors:  Yiyuan Yin; Yili Li; Melissa C Kerzic; Roland Martin; Roy A Mariuzza
Journal:  EMBO J       Date:  2011-02-04       Impact factor: 11.598

4.  A critical role for conserved residues in the cleft of HLA-A2 in presentation of a nonapeptide to T cells.

Authors:  F Latron; L Pazmany; J Morrison; R Moots; M A Saper; A McMichael; J L Strominger
Journal:  Science       Date:  1992-08-14       Impact factor: 47.728

5.  How to measure and predict the molar absorption coefficient of a protein.

Authors:  C N Pace; F Vajdos; L Fee; G Grimsley; T Gray
Journal:  Protein Sci       Date:  1995-11       Impact factor: 6.725

6.  The triple helix in equilibrium with coil conversion of collagen-like polytripeptides in aqueous and nonaqueous solvents. Comparison of the thermodynamic parameters and the binding of water to (L-Pro-L-Pro-Gly)n and (L-Pro-L-Hyp-Gly)n.

Authors:  J Engel; H T Chen; D J Prockop; H Klump
Journal:  Biopolymers       Date:  1977-03       Impact factor: 2.505

7.  Structural Mechanism Underpinning Cross-reactivity of a CD8+ T-cell Clone That Recognizes a Peptide Derived from Human Telomerase Reverse Transcriptase.

Authors:  David K Cole; Hugo A van den Berg; Angharad Lloyd; Michael D Crowther; Konrad Beck; Julia Ekeruche-Makinde; John J Miles; Anna M Bulek; Garry Dolton; Andrea J Schauenburg; Aaron Wall; Anna Fuller; Mathew Clement; Bruno Laugel; Pierre J Rizkallah; Linda Wooldridge; Andrew K Sewell
Journal:  J Biol Chem       Date:  2016-11-30       Impact factor: 5.157

8.  Human TCR-binding affinity is governed by MHC class restriction.

Authors:  David K Cole; Nicholas J Pumphrey; Jonathan M Boulter; Malkit Sami; John I Bell; Emma Gostick; David A Price; George F Gao; Andrew K Sewell; Bent K Jakobsen
Journal:  J Immunol       Date:  2007-05-01       Impact factor: 5.422

9.  Structural basis for the killing of human beta cells by CD8(+) T cells in type 1 diabetes.

Authors:  Anna M Bulek; David K Cole; Ania Skowera; Garry Dolton; Stephanie Gras; Florian Madura; Anna Fuller; John J Miles; Emma Gostick; David A Price; Jan W Drijfhout; Robin R Knight; Guo C Huang; Nikolai Lissin; Peter E Molloy; Linda Wooldridge; Bent K Jakobsen; Jamie Rossjohn; Mark Peakman; Pierre J Rizkallah; Andrew K Sewell
Journal:  Nat Immunol       Date:  2012-01-15       Impact factor: 25.606

10.  Hotspot autoimmune T cell receptor binding underlies pathogen and insulin peptide cross-reactivity.

Authors:  David K Cole; Anna M Bulek; Garry Dolton; Andrea J Schauenberg; Barbara Szomolay; William Rittase; Andrew Trimby; Prithiviraj Jothikumar; Anna Fuller; Ania Skowera; Jamie Rossjohn; Cheng Zhu; John J Miles; Mark Peakman; Linda Wooldridge; Pierre J Rizkallah; Andrew K Sewell
Journal:  J Clin Invest       Date:  2016-05-16       Impact factor: 14.808
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  2 in total

1.  Dual Molecular Mechanisms Govern Escape at Immunodominant HLA A2-Restricted HIV Epitope.

Authors:  David K Cole; Anna Fuller; Garry Dolton; Efthalia Zervoudi; Mateusz Legut; Kim Miles; Lori Blanchfield; Florian Madura; Christopher J Holland; Anna M Bulek; John S Bridgeman; John J Miles; Andrea J A Schauenburg; Konrad Beck; Brian D Evavold; Pierre J Rizkallah; Andrew K Sewell
Journal:  Front Immunol       Date:  2017-11-10       Impact factor: 7.561

2.  T cell receptor alpha variable 12-2 bias in the immunodominant response to Yellow fever virus.

Authors:  Amandine Bovay; Vincent Zoete; Garry Dolton; Anna M Bulek; David K Cole; Pierre J Rizkallah; Anna Fuller; Konrad Beck; Olivier Michielin; Daniel E Speiser; Andrew K Sewell; Silvia A Fuertes Marraco
Journal:  Eur J Immunol       Date:  2017-12-11       Impact factor: 5.532
  2 in total

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