Literature DB >> 2874805

Preparation and binding of radioactively labeled porcine transforming growth factor type beta.

E Ruff, A Rizzino.   

Abstract

This report describes the labeling of porcine transforming growth factor type beta (TGF-beta) with 125-iodine. Its binding to NRK cells and three other cell lines has been examined. The data indicate that NRK cells exhibit approximately 10,000 receptors for porcine TGF-beta per cell, with an apparent dissociation constant of 45 pM. The binding of porcine 125I-TGF-beta can be blocked by porcine, murine and human TGF-beta but not by several well characterized growth factors. In all respects examined, the binding observed with porcine 125I-TGF-beta appears to be the same as that observed with human TGF-beta. The findings reported here argue that porcine 125I-TGF-beta can be used to quantitate TGF-beta receptors on a wide range of mammalian cells.

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Year:  1986        PMID： 2874805     DOI： 10.1016/s0006-291x(86)80555-1

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  13 in total

1.  Isolation and characterization of mutant cell lines defective in transforming growth factor beta signaling.

Authors:  B A Hocevar; P H Howe
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-23       Impact factor: 11.205

2.  Density-induced down regulation of epidermal growth factor receptors.

Authors:  A Rizzino; P Kazakoff; J Nebelsick
Journal:  In Vitro Cell Dev Biol       Date:  1990-05

3.  A 16-amino acid peptide from human alpha2-macroglobulin binds transforming growth factor-beta and platelet-derived growth factor-BB.

Authors:  D J Webb; D W Roadcap; A Dhakephalkar; S L Gonias
Journal:  Protein Sci       Date:  2000-10       Impact factor: 6.725

4.  Proteinases are isoform-specific regulators of the binding of transforming growth factor beta to alpha 2-macroglobulin.

Authors:  D J Webb; A M Weaver; T L Atkins-Brady; S L Gonias
Journal:  Biochem J       Date:  1996-12-01       Impact factor: 3.857

5.  Differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.

Authors:  S Y Koyama; D K Podolsky
Journal:  J Clin Invest       Date:  1989-05       Impact factor: 14.808

6.  Binding of platelet-derived growth factor-BB and transforming growth factor-beta 1 to alpha 2-macroglobulin in vitro and in vivo: comparison of receptor-recognized and non-recognized alpha 2-macroglobulin conformations.

Authors:  K P Crookston; D J Webb; J Lamarre; S L Gonias
Journal:  Biochem J       Date:  1993-07-15       Impact factor: 3.857

7.  Characterization of transforming growth factor-beta (TGF-beta) receptors on BeWo choriocarcinoma cells including the identification of a novel 38-kDa TGF-beta binding glycoprotein.

Authors:  E J Mitchell; K Lee; M D O'Connor-McCourt
Journal:  Mol Biol Cell       Date:  1992-11       Impact factor: 4.138

8.  Cytosine arabinoside increases the binding of 125I-labelled epidermal growth factor and 125I-transferrin and enhances the in vitro targeting of human tumour cells with anti-(growth factor receptor) mAb.

Authors:  M Caraglia; P Tagliaferri; P Correale; G Genua; A Pinto; S Del Vecchio; G Esposito; A R Bianco
Journal:  Cancer Immunol Immunother       Date:  1993-08       Impact factor: 6.968

9.  Differences in the binding of transforming growth factor beta 1 to the acute-phase reactant and constitutively synthesized alpha-macroglobulins of rat.

Authors:  D J Webb; K P Crookston; N L Figler; J Lamarre; S L Gonias
Journal:  Biochem J       Date:  1995-12-01       Impact factor: 3.857

10.  Binding of transforming growth factor-beta 1 to methylamine-modified alpha 2-macroglobulin and to binary and ternary alpha 2-macroglobulin-proteinase complexes.

Authors:  S W Hall; J LaMarre; L B Marshall; M A Hayes; S L Gonias
Journal:  Biochem J       Date:  1992-01-15       Impact factor: 3.857
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