Nariman Shahhosseini1, Sadegh Chinikar2, Seyed Hassan Moosa-Kazemi3, Mohammad Mehdi Sedaghat3, Mohammad Hassan Kayedi4, Renke Lühken1, Jonas Schmidt-Chanasit1,5. 1. Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany. 2. Pasteur Institute of Tehran, Tehran, Iran. 3. Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 4. Department of Parasitology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 5. German Centre for Infection Research (DZIF), Partner site Hamburg-Luebeck-Borstel, Hamburg, Germany.
Abstract
OBJECTIVE: Screening of mosquitoes for viruses is an important forecasting tool for emerging and re-emerging arboviruses. Iran has been known to harbour medically important arboviruses such as West Nile virus (WNV) and dengue virus (DENV) based on seroepidemiological data. However, there are no data about the potential mosquito vectors for arboviruses in Iran. This study was performed to provide mosquito and arbovirus data from Iran. MATERIALS AND METHODS: A total of 32 317 mosquitos were collected at 16 sites in five provinces of Iran in 2015 and 2016. RT-PCR for detection of flaviviruses was performed. The PCR amplicons were sequenced, and 109 WNV sequences, including one obtained in this study, were used for phylogenetic analyses. RESULTS: The 32 317 mosquito specimens belonging to 25 species were morphologically distinguished and distributed into 1222 pools. Culex pipiens s.l. comprised 56.429%. One mosquito pool (0.08%), containing 46 unfed Cx. pipiens pipiens form pipiens (Cpp) captured in August 2015, was positive for flavivirus RNA. Subsequent sequencing and phylogenetic analyses revealed that the detected Iranian WNV strain belongs to lineage 2 and clusters with a strain recently detected in humans. No flaviviruses other than WNV were detected in the mosquito pools. CONCLUSION: Cpp could be a vector for WNV in Iran. Our findings indicate recent circulation of WNV lineage-2 strain in Iran and provide a solid base for more targeted arbovirus surveillance programs.
OBJECTIVE: Screening of mosquitoes for viruses is an important forecasting tool for emerging and re-emerging arboviruses. Iran has been known to harbour medically important arboviruses such as West Nile virus (WNV) and dengue virus (DENV) based on seroepidemiological data. However, there are no data about the potential mosquito vectors for arboviruses in Iran. This study was performed to provide mosquito and arbovirus data from Iran. MATERIALS AND METHODS: A total of 32 317 mosquitos were collected at 16 sites in five provinces of Iran in 2015 and 2016. RT-PCR for detection of flaviviruses was performed. The PCR amplicons were sequenced, and 109 WNV sequences, including one obtained in this study, were used for phylogenetic analyses. RESULTS: The 32 317 mosquito specimens belonging to 25 species were morphologically distinguished and distributed into 1222 pools. Culex pipiens s.l. comprised 56.429%. One mosquito pool (0.08%), containing 46 unfed Cx. pipienspipiens form pipiens (Cpp) captured in August 2015, was positive for flavivirus RNA. Subsequent sequencing and phylogenetic analyses revealed that the detected Iranian WNV strain belongs to lineage 2 and clusters with a strain recently detected in humans. No flaviviruses other than WNV were detected in the mosquito pools. CONCLUSION: Cpp could be a vector for WNV in Iran. Our findings indicate recent circulation of WNV lineage-2 strain in Iran and provide a solid base for more targeted arbovirus surveillance programs.
Authors: Nariman Shahhosseini; Mohammad Hassan Kayedi; Mohammad Mehdi Sedaghat; Trina Racine; Gary P Kobinger; Seyed Hassan Moosa-Kazemi Journal: PLoS One Date: 2018-11-14 Impact factor: 3.240
Authors: Sana Eybpoosh; Mehdi Fazlalipour; Vahid Baniasadi; Mohammad Hassan Pouriayevali; Farzin Sadeghi; Abbas Ahmadi Vasmehjani; Mohammad Hadi Karbalaie Niya; Roger Hewson; Mostafa Salehi-Vaziri Journal: PLoS Negl Trop Dis Date: 2019-01-29
Authors: Nariman Shahhosseini; Ahmad Jafarbekloo; Zakkyeh Telmadarraiy; Sadegh Chinikar; Ali Haeri; Norbert Nowotny; Martin H Groschup; Anthony R Fooks; Faezeh Faghihi Journal: Heliyon Date: 2017-11-13