Junjun Chen1, Guanhuan Du2, Yufeng Wang2, Linjun Shi2, Jun Mi3, Guoyao Tang4. 1. Department of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, China. 2. Department of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. 3. Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, China. 4. Department of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: Tanggy@shsmu.edu.cn.
Abstract
OBJECTIVE: Oral lichen planus (OLP), a chronic inflammatory disease of unknown etiology, is considered a potentially malignant oral disorder. The aim of the present study was to analyze candidate microRNAs (miRNAs) and genes from patients with OLP and healthy controls (HCs). STUDY DESIGN: Biopsy specimens of the oral mucosa were collected from patients with OLP (n = 9) and from HCs (n = 4). Differentially expressed miRNAs and differentially expressed genes were screened by using next-generation sequencing with DESeq and edgeR software algorithms. RESULTS: A total of 94 differentially expressed miRNAs and 599 differentially expressed genes were detected in OLP. Potential regulatory miRNAs and genes were obtained by analyzing miRNA-messenger RNA networks. Of these, 5 downregulated miRNAs-Hsa-miR-135 a-5 p (P = .33), hsa-miR-128-3 p (P = .03), hsa-miR-218-5 p (P = .01), hsa-miR-125 a-5 p (P = .01), and hsa-let-7 e-5 p (P = .04)-were the most promising biomarkers in patients with OLP compared with HCs. The identified differentially expressed genes were significantly enriched in "inflammatory" events and immune-related terms through Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis. CONCLUSIONS: The integrative analysis of messenger RNA and miRNA profiles provides important information to elucidate gene expression mechanisms and a comprehensive perspective to study the etiology and pathogenesis of OLP.
OBJECTIVE: Oral lichen planus (OLP), a chronic inflammatory disease of unknown etiology, is considered a potentially malignant oral disorder. The aim of the present study was to analyze candidate microRNAs (miRNAs) and genes from patients with OLP and healthy controls (HCs). STUDY DESIGN: Biopsy specimens of the oral mucosa were collected from patients with OLP (n = 9) and from HCs (n = 4). Differentially expressed miRNAs and differentially expressed genes were screened by using next-generation sequencing with DESeq and edgeR software algorithms. RESULTS: A total of 94 differentially expressed miRNAs and 599 differentially expressed genes were detected in OLP. Potential regulatory miRNAs and genes were obtained by analyzing miRNA-messenger RNA networks. Of these, 5 downregulated miRNAs-Hsa-miR-135 a-5 p (P = .33), hsa-miR-128-3 p (P = .03), hsa-miR-218-5 p (P = .01), hsa-miR-125 a-5 p (P = .01), and hsa-let-7 e-5 p (P = .04)-were the most promising biomarkers in patients with OLP compared with HCs. The identified differentially expressed genes were significantly enriched in "inflammatory" events and immune-related terms through Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis. CONCLUSIONS: The integrative analysis of messenger RNA and miRNA profiles provides important information to elucidate gene expression mechanisms and a comprehensive perspective to study the etiology and pathogenesis of OLP.
Authors: Mir S Adil; Varun Parvathagiri; Arti Verma; Fang Liu; Madhuri Rudraraju; S Priya Narayanan; Payaningal R Somanath Journal: Cells Date: 2022-05-29 Impact factor: 7.666