Cheng-Mei Yang1, Chien-Chou Chen2, Yu-Kai Tseng3, Sin-Jhih Huang4, Huei-Han Liou4, Yi-Cheng Lee5, Jang-Hwa Lee6, Jyh-Seng Wang7, Hung-Chih Chen8, Chao-Chuan Chi9, Bor-Hwang Kang10, Yun-Chung Lin11, Kuo-Wang Tsai12, Luo-Ping Ger13. 1. Director, Division of Endodontics, Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; Assistant Professor, Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan. 2. Director, Department of Family Medicine, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan. 3. Resident Doctor, Department of Orthopedics, Show Chwan Memorial Hospital, Changhua, Taiwan; Resident Doctor, Department of Orthopedics, National Cheng Kung University Hospital, Tainan, Taiwan. 4. Research Assistant, Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 5. Research Assistant, Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; Registered Nurse, Department of Nursing, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 6. Director, Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 7. Attending Doctor, Department of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 8. Assistant Professor, Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan; Director, Division of Oral & Maxillary surgery, Department of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 9. Director, Division of Laryngology, Department of Otorhinolaryngology-Head & Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 10. Director, Division of Rhinology, Department of Otorhinolaryngology-Head & Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. 11. Resident Doctor, Department of Pathology, China Medical University Hospital, Taichung, Taiwan. 12. Investigator, Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; Assistant Professor, Department of Chemical Biology, National Pingtung University of Education, Pingtung, Taiwan. Electronic address: Kwtsai@vghks.gov.tw. 13. Investigator, Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; Professor, Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan. Electronic address: Lpger0329@gmail.com.
Abstract
OBJECTIVES: This case-control study evaluated the association of the single nucleotide polymorphism rs7372209 (T>C) in pri-mir-26a-1 with the risk and progression of betel quid (BQ)-related oral premalignant lesions (OPLs) and oral squamous cell carcinoma (OSCC). STUDY DESIGN: In total, 597 BQ chewers were recruited: 196 healthy controls, 241 patients with OPLs, and 160 patients with OSCC. Genotypes were determined using the TaqMan real-time assay. RESULTS: The C/T + T/T genotypes and T allele in pri-mir-26a-1 were correlated with a decreased risk of BQ-related OPLs (P = .038 and .005, respectively), oral leukoplakia (P = .01 and .001, respectively), and advanced-stage OSCC (P = .021 and .004, respectively). The effects of the C/T + T/T genotypes and T allele on the decreased risk of OPLs were potent in the older age group (both Pinteraction < .001), heavy smokers (Pinteraction ≤ .003 and .006, respectively) and alcohol drinkers (Pinteraction ≤ .004 and .001, respectively). Furthermore, among patients with OSCC, the C/T + T/T genotypes and T allele were associated with a decreased risk of advanced pathologic stage (P = .032) and lymph node involvement (P = .017). CONCLUSIONS: BQ chewers carrying the T allele or C/T + T/T genotypes in pri-mir-26a-1 may have a decreased risk of oral leukoplakia, OPLs, and advanced-stage OSCC.
OBJECTIVES: This case-control study evaluated the association of the single nucleotide polymorphism rs7372209 (T>C) in pri-mir-26a-1 with the risk and progression of betel quid (BQ)-related oral premalignant lesions (OPLs) and oral squamous cell carcinoma (OSCC). STUDY DESIGN: In total, 597 BQ chewers were recruited: 196 healthy controls, 241 patients with OPLs, and 160 patients with OSCC. Genotypes were determined using the TaqMan real-time assay. RESULTS: The C/T + T/T genotypes and T allele in pri-mir-26a-1 were correlated with a decreased risk of BQ-related OPLs (P = .038 and .005, respectively), oral leukoplakia (P = .01 and .001, respectively), and advanced-stage OSCC (P = .021 and .004, respectively). The effects of the C/T + T/T genotypes and T allele on the decreased risk of OPLs were potent in the older age group (both Pinteraction < .001), heavy smokers (Pinteraction ≤ .003 and .006, respectively) and alcohol drinkers (Pinteraction ≤ .004 and .001, respectively). Furthermore, among patients with OSCC, the C/T + T/T genotypes and T allele were associated with a decreased risk of advanced pathologic stage (P = .032) and lymph node involvement (P = .017). CONCLUSIONS: BQ chewers carrying the T allele or C/T + T/T genotypes in pri-mir-26a-1 may have a decreased risk of oral leukoplakia, OPLs, and advanced-stage OSCC.