Literature DB >> 28740311

Antimicrobial resistance and typing of Salmonella isolated from street vended foods and associated environment.

Bi Shagufta1, M Sivakumar1, Surender Kumar1, Rajesh Kumar Agarwal1, Kiran Narayan Bhilegaonkar1, Ashok Kumar1, Zunjar Baburao Dubal1.   

Abstract

The present study was carried out to find out the occurrence and types of Salmonella present in street vended foods and associated environment, and their resistance pattern against various antibiotics. About 1075 street vended food and associated environment samples were processed for isolation and confirmation of different Salmonella spp. by targeting gene specific invA gene and serotype specific Sdf I, Via B and Spy genes by PCR. Selected Salmonella isolates were screened for antibiotic resistance by using Baeur-Kirby disk diffusion test. Out of 1075 samples, only 31 (2.88%) isolates could be amplified the invA gene of which 19 could be recovered from meat vendors; 8 from egg vendors while remaining 4 from milk vendors. Though, majority of Salmonella recovered from raw foods the ready-to-eat food like chicken gravy and rasmalai also showed its presence which pose a serious public health threat. Overall, 19, 6 and 1 isolates of S. Typhimurium, S. Enteritidis and S. Typhi could be detected by PCR while remaining 5 isolates could not be amplified suggesting other type of Salmonella. Selected Salmonella isolates were completely resistance to Oxacillin (100%) followed by Cefoxitin (30.43%) and Ampicillin (26.10%). Thus, it is observed that the street vended foods of animal origin and associated environment play an important role in transmission of food borne pathogens including Salmonella.

Entities:  

Keywords:  Antibiotic resistance; Salmonella; Street food; Typing

Year:  2017        PMID: 28740311      PMCID: PMC5502049          DOI: 10.1007/s13197-017-2698-1

Source DB:  PubMed          Journal:  J Food Sci Technol        ISSN: 0022-1155            Impact factor:   2.701


  25 in total

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Authors:  E J Threlfall; L R Ward; J A Frost; G A Willshaw
Journal:  Int J Food Microbiol       Date:  2000-12-05       Impact factor: 5.277

2.  Characterization of south african isolates of Salmonella enteritidis by phage typing, numerical analysis of RAPD-PCR banding patterns and plasmid profiles.

Authors:  L Maré; L M Dick; M L van der Walt
Journal:  Int J Food Microbiol       Date:  2001-03-20       Impact factor: 5.277

3.  Genomics-based food-borne pathogen testing and diagnostics: possibilities for the U.S. Department of Agriculture's Food Safety and Inspection Service.

Authors:  James Withee; Kerry L Dearfield
Journal:  Environ Mol Mutagen       Date:  2007-06       Impact factor: 3.216

4.  Antibiotic resistance pattern among the Salmonella isolated from human, animal and meat in India.

Authors:  Shweta Singh; Rajesh Kumar Agarwal; Suresh C Tiwari; Himanshu Singh
Journal:  Trop Anim Health Prod       Date:  2011-08-20       Impact factor: 1.559

5.  Population structure of Salmonella enterica serovar 4,[5],12:b:- strains and likely sources of human infection.

Authors:  Anne Toboldt; Erhard Tietze; Reiner Helmuth; Ernst Junker; Angelika Fruth; Burkhard Malorny
Journal:  Appl Environ Microbiol       Date:  2013-06-21       Impact factor: 4.792

6.  The global burden of nontyphoidal Salmonella gastroenteritis.

Authors:  Shannon E Majowicz; Jennie Musto; Elaine Scallan; Frederick J Angulo; Martyn Kirk; Sarah J O'Brien; Timothy F Jones; Aamir Fazil; Robert M Hoekstra
Journal:  Clin Infect Dis       Date:  2010-03-15       Impact factor: 9.079

7.  Detection of Salmonella enterica serovar Typhi (S. Typhi) by selective amplification of invA, viaB, fliC-d and prt genes by polymerase chain reaction in mutiplex format.

Authors:  S Kumar; K Balakrishna; H V Batra
Journal:  Lett Appl Microbiol       Date:  2006-02       Impact factor: 2.858

Review 8.  Emerging food-borne zoonoses.

Authors:  J Schlundt; H Toyofuku; J Jansen; S A Herbst
Journal:  Rev Sci Tech       Date:  2004-08       Impact factor: 1.181

9.  Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella.

Authors:  K Rahn; S A De Grandis; R C Clarke; S A McEwen; J E Galán; C Ginocchio; R Curtiss; C L Gyles
Journal:  Mol Cell Probes       Date:  1992-08       Impact factor: 2.365

10.  Simple and rapid detection of Salmonella strains by direct PCR amplification of the hilA gene.

Authors:  S G Pathmanathan; N Cardona-Castro; M M Sánchez-Jiménez; M M Correa-Ochoa; S D Puthucheary; K L Thong
Journal:  J Med Microbiol       Date:  2003-09       Impact factor: 2.472

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