Syeda Kiran Riaz1, Lin Ye2, Namood E Sahar1, Durkhshan Aman1, Javeria Qadir1, Jahangir Sarwar Khan3, Muhammad Saeed1, Wen G Jiang3, Muhammad Faraz Arshad Malik4. 1. Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan. 2. Department of Surgery, Holy Family Hospital, Rawalpindi Medical College, Rawalpindi, Pakistan. 3. Cardiff China Medical Research Collaborative, School of Medicine, Cardiff University, Cardiff, U.K. 4. Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan famalik@comsats.edu.pk.
Abstract
BACKGROUND/AIM: Constitutive activation of Sonic hedgehog (SHH) has been observed in different types of cancers. In the present study, expressional profiling of SHH in a breast cancer cohort (n=150) of a Pakistani population and its association with different molecular subtypes have been explored. MATERIALS AND METHODS: qRT-PCR and IHC were performed for expression analysis of SHH and its association with ER, PR, HER2 and Ki-67 were also statistically analyzed. RESULTS: A significant over-expression of SHH was observed in tumor tissues in comparison to their respective controls (p<0.0001). A strong positive correlation was seen between SHH and proliferation marker (r=0.635, p=0). SHH expression was significantly high among patients with advanced tumor grade, stage, nodal involvement and metastasis. Furthermore, both luminal-B and triple-negative subtypes of cohort showed increased expression of SHH. CONCLUSION: Based on these findings, SHH may be used as a potential biomarker for breast carcinogenesis. Copyright
BACKGROUND/AIM: Constitutive activation of Sonic hedgehog (SHH) has been observed in different types of cancers. In the present study, expressional profiling of SHH in a breast cancer cohort (n=150) of a Pakistani population and its association with different molecular subtypes have been explored. MATERIALS AND METHODS: qRT-PCR and IHC were performed for expression analysis of SHH and its association with ER, PR, HER2 and Ki-67 were also statistically analyzed. RESULTS: A significant over-expression of SHH was observed in tumor tissues in comparison to their respective controls (p<0.0001). A strong positive correlation was seen between SHH and proliferation marker (r=0.635, p=0). SHH expression was significantly high among patients with advanced tumor grade, stage, nodal involvement and metastasis. Furthermore, both luminal-B and triple-negative subtypes of cohort showed increased expression of SHH. CONCLUSION: Based on these findings, SHH may be used as a potential biomarker for breast carcinogenesis. Copyright