Zhaohua Gong1, Cheng Chi1, Xiaojuan Huang2, Hongjin Chu3, Jiahui Wang3, Fengcai Du1, Lixin Jiang4, Jian Chen5,3. 1. Department of Oncology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China. 2. First Department of Chemotherapy, Weihai Municipal Hospital, Weihai, P.R. China. 3. The Central Laboratory, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China. 4. Department of Gastrointestinal Surgery, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China. 5. Department of Oncology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China chenjianyt@163.com.
Abstract
AIM: To investigate the expression of cyclophilin A (CypA) in human hepatocellular carcinoma (HCC) and explore the effects of CypA on the cell cycle in HCC. MATERIALS AND METHODS: CypA expression was assessed by immunohistochemistry in 48 cases of HCC tissues and paired adjacent tissues. CypA plasmid was transfected into HCC cells and the cell cycle was analyzed. RESULTS: Positivity for CypA was higher in HCC tissues than in adjacent tissues (79.1% vs. 12.5%, p<0.05). Positivity for CypA was significantly higher in stage III and IV HCC than in stage I and II (p<0.05). Elevated CypA induced an increase of the percentage of S-phase cells (from 34.79% to 42.14%) and a decrease of G0-G1 phase cells (from 58.10% to 50.64%). CONCLUSION: CypA is overexpressed in HCC and is associated with TNM stage. CypA also appears to promote the transition of the cell cycle from G1 to S phase. Copyright
AIM: To investigate the expression of cyclophilin A (CypA) in humanhepatocellular carcinoma (HCC) and explore the effects of CypA on the cell cycle in HCC. MATERIALS AND METHODS:CypA expression was assessed by immunohistochemistry in 48 cases of HCC tissues and paired adjacent tissues. CypA plasmid was transfected into HCC cells and the cell cycle was analyzed. RESULTS: Positivity for CypA was higher in HCC tissues than in adjacent tissues (79.1% vs. 12.5%, p<0.05). Positivity for CypA was significantly higher in stage III and IV HCC than in stage I and II (p<0.05). Elevated CypA induced an increase of the percentage of S-phase cells (from 34.79% to 42.14%) and a decrease of G0-G1 phase cells (from 58.10% to 50.64%). CONCLUSION:CypA is overexpressed in HCC and is associated with TNM stage. CypA also appears to promote the transition of the cell cycle from G1 to S phase. Copyright
Authors: Rui Hao; He Lu; Yanan Guo; Qianqian Liu; Lu Wang; Yang Wang; Ailong Huang; Zeng Tu Journal: Medicine (Baltimore) Date: 2021-08-20 Impact factor: 1.817