Sarai Estrella Sandoval-Azuara1, Raquel Muñiz-Salazar2, Ricardo Perea-Jacobo3, Suelee Robbe-Austerman4, Alejandro Perera-Ortiz5, Gilberto López-Valencia6, Doris M Bravo7, Alejandro Sanchez-Flores8, Daniela Miranda-Guzmán9, Carlos Alberto Flores-López10, Roberto Zenteno-Cuevas11, Rafael Laniado-Laborín12, Fabiola Lafarga de la Cruz13, Tod P Stuber14. 1. Laboratorio de Epidemiología y Ecología Molecular, Escuela Ciencias de la Salud, Universidad Autónoma de Baja California, Blvd Zertuche y Blvd de los Lagos, Fracc, Valle Dorado, CP 22890, Ensenada, Baja California, Mexico; Facultad de Ciencias Marinas, Universidad Autónoma de Baja California,Tijuana - Ensenada road 103 km, CP 22860, Ensenada, Baja California, Mexico. Electronic address: estrella.sandoval@uabc.edu.mx. 2. Laboratorio de Epidemiología y Ecología Molecular, Escuela Ciencias de la Salud, Universidad Autónoma de Baja California, Blvd Zertuche y Blvd de los Lagos, Fracc, Valle Dorado, CP 22890, Ensenada, Baja California, Mexico. Electronic address: ramusal@uabc.edu.mx. 3. Laboratorio de Epidemiología y Ecología Molecular, Escuela Ciencias de la Salud, Universidad Autónoma de Baja California, Blvd Zertuche y Blvd de los Lagos, Fracc, Valle Dorado, CP 22890, Ensenada, Baja California, Mexico; Facultad de Ciencias Marinas, Universidad Autónoma de Baja California,Tijuana - Ensenada road 103 km, CP 22860, Ensenada, Baja California, Mexico. Electronic address: perear@uabc.edu.mx. 4. National Veterinary Services Laboratories, United States Department of Agriculture, 1015 N University Blvd, Ames, IA 50011, USA. Electronic address: Suelee.Robbe-Austerman@aphis.usda.gov. 5. United States Department of Agriculture, Animal and Plant Health Inspection Service, Mexico. Electronic address: Alejandro.Perera@aphis.usda.gov. 6. Instituto de Investigaciones en Ciencias Veterinarias, Laboratorio de Tuberculosis y Brucelosis, Universidad Autónoma de Baja California, San Felipe road 3.5 km, Fracc, Campestre, CP 21386, Mexicali, Baja California, Mexico. Electronic address: gilbertolopez@uabc.edu.mx. 7. National Veterinary Services Laboratories, United States Department of Agriculture, 1015 N University Blvd, Ames, IA 50011, USA. Electronic address: doris.m.bravo@aphis.usda.gov. 8. Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, UNAM, Av. Universidad 2001, Chamilpa, CP 62210, Cuernavaca, Morelos, Mexico. Electronic address: alexsf@ibt.unam.mx. 9. Instituto de Investigaciones en Ciencias Veterinarias, Laboratorio de Tuberculosis y Brucelosis, Universidad Autónoma de Baja California, San Felipe road 3.5 km, Fracc, Campestre, CP 21386, Mexicali, Baja California, Mexico. Electronic address: mvz.daniela.miranda@gmail.com. 10. Facultad de Ciencias, Universidad Autónoma de Baja California, Tijuana - Ensenada road 103 km, CP 22860, Ensenada, Baja California, Mexico. Electronic address: cflores2@uabc.edu.mx. 11. Instituto de Salud Pública, Universidad Veracruzana, Doctor Luis Castelazso, Industrial Aniimas, CP 91190, Xalapa Enríquez, Veracruz, Mexico. Electronic address: robzencue@gmail.com. 12. Clínica de Tuberculosis, Hospital General de Tijuana, ISESALUD, Av. Centenario 10851, Zona Rio, CP 22680, Tijuana, Baja California, Mexico. Electronic address: rlaniado@uabc.edu.mx. 13. Departamento de Acuicultura, Centro de Investigación Científica y de Educación Superación de Ensenada, Carretera Ensenada-Tijuana No. 3918, Zona Playitas, CP 22860, Ensenada, Baja California, Mexico. Electronic address: flafarga@cicese.mx. 14. National Veterinary Services Laboratories, United States Department of Agriculture, 1015 N University Blvd, Ames, IA 50011, USA. Electronic address: Tod.P.Stuber@aphis.usda.gov.
Abstract
OBJECTIVES: To determine genetic diversity by comparing the whole genome sequences of cattle and human Mycobacterium bovis isolates from Baja California. METHODS: A whole genome sequencing strategy was used to obtain the molecular fingerprints of 172 isolates of M. bovis obtained from Baja California, Mexico; 155 isolates were from cattle and 17 isolates were from humans. Spoligotypes were characterized in silico and single nucleotide polymorphism (SNP) differences between the isolates were evaluated. RESULTS: A total of 12 M. bovis spoligotype patterns were identified in cattle and humans. Two predominant spoligotypes patterns were seen in both cattle and humans: SB0145 and SB1040. The SB0145 spoligotype represented 59% of cattle isolates (n=91) and 65% of human isolates (n=11), while the SB1040 spoligotype represented 30% of cattle isolates (n=47) and 30% of human isolates (n=5). When evaluating SNP differences, the human isolates were intimately intertwined with the cattle isolates. CONCLUSIONS: All isolates from humans had spoligotype patterns that matched those observed in the cattle isolates, and all human isolates shared common ancestors with cattle in Baja California based on SNP analysis. This suggests that most human tuberculosis caused by M. bovis in Baja California is derived from M. bovis circulating in Baja California cattle. These results reinforce the importance of bovine tuberculosis surveillance and control in this region.
OBJECTIVES: To determine genetic diversity by comparing the whole genome sequences of cattle and humanMycobacterium bovis isolates from Baja California. METHODS: A whole genome sequencing strategy was used to obtain the molecular fingerprints of 172 isolates of M. bovis obtained from Baja California, Mexico; 155 isolates were from cattle and 17 isolates were from humans. Spoligotypes were characterized in silico and single nucleotide polymorphism (SNP) differences between the isolates were evaluated. RESULTS: A total of 12 M. bovis spoligotype patterns were identified in cattle and humans. Two predominant spoligotypes patterns were seen in both cattle and humans: SB0145 and SB1040. The SB0145 spoligotype represented 59% of cattle isolates (n=91) and 65% of human isolates (n=11), while the SB1040 spoligotype represented 30% of cattle isolates (n=47) and 30% of human isolates (n=5). When evaluating SNP differences, the human isolates were intimately intertwined with the cattle isolates. CONCLUSIONS: All isolates from humans had spoligotype patterns that matched those observed in the cattle isolates, and all human isolates shared common ancestors with cattle in Baja California based on SNP analysis. This suggests that most humantuberculosis caused by M. bovis in Baja California is derived from M. bovis circulating in Baja California cattle. These results reinforce the importance of bovinetuberculosis surveillance and control in this region.
Authors: Marian Price-Carter; Rudiger Brauning; Geoffrey W de Lisle; Paul Livingstone; Mark Neill; Jane Sinclair; Brent Paterson; Gillian Atkinson; Garry Knowles; Kevin Crews; Joseph Crispell; Rowland Kao; Suelee Robbe-Austerman; Tod Stuber; Julian Parkhill; James Wood; Simon Harris; Desmond M Collins Journal: Front Vet Sci Date: 2018-10-30
Authors: Nina Van Goethem; Tine Descamps; Brecht Devleesschauwer; Nancy H C Roosens; Nele A M Boon; Herman Van Oyen; Annie Robert Journal: Implement Sci Date: 2019-08-13 Impact factor: 7.327
Authors: Cristina Kraemer Zimpel; José Salvatore L Patané; Aureliano Coelho Proença Guedes; Robson F de Souza; Taiana T Silva-Pereira; Naila C Soler Camargo; Antônio F de Souza Filho; Cássia Y Ikuta; José Soares Ferreira Neto; João Carlos Setubal; Marcos Bryan Heinemann; Ana Marcia Sa Guimaraes Journal: Front Microbiol Date: 2020-05-07 Impact factor: 5.640
Authors: Andries J van Tonder; Mark J Thornton; Andrew J K Conlan; Keith A Jolley; Lee Goolding; Andrew P Mitchell; James Dale; Eleftheria Palkopoulou; Philip J Hogarth; R Glyn Hewinson; James L N Wood; Julian Parkhill Journal: PLoS Pathog Date: 2021-11-29 Impact factor: 6.823