Rawoof Malik1, Rabia Farooq2, Promela Mehta1, Sheikh Ishaq1, Insha Din1, Parvaiz Shah3, Sabhiya Majid4. 1. Department of Biochemistry, Govt Medical College Srinagar Kashmir, J and K, India. 2. Department of Human Genetics, Punjabi University, Patiala, Punjab, India. 3. Department of Medicine, Government Medical College, Srinagar Kashmir, J and K, India. 4. Department of Biochemistry, Govt Medical College Srinagar Kashmir, J and K, India. Electronic address: sabumajid11@gmail.com.
Abstract
OBJECTIVES: Approx 1 billion people across various ethnic and age groups have vitamin D deficiency. The high prevalence of such a deficiency is an imperative public health issue because hypovitaminosis D is an autonomous risk factor for mortality in the general population. Beyond bone integrity and calcium homeostasis, it is involved in numerous physiologic and pathologic processes. The role of vitamin D in the pathogenesis and prevention of type 2 diabetes mellitus has sparked universal interest. METHODS: This hospital-based case-control study was designed to study the association between 25-hydroxy vitamin D (25[OH]D) levels and the vitamin D receptor (VDR) gene polymorphism with diabetes and to evaluate their roles as risk factors for diabetes. 100 cases and controls were taken. 25(OH)D levels were analyzed by the chemilumenescence method using a Siemens ADVIA Centaur analyzer. Genomic DNA was extracted and Taq-1 and Bsm-1 genotyping in the VDR gene was done by using the polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP). RESULTS: 25(OH)D levels of patients with diabetes were significantly lower than those of controls (19.26±0.95 ng/mL vs. 25.49±1.02 ng/mL; p=0.001). 25(OH)D levels were found to be inversely associated with glycated hemoglobin percentages in cases (r2=0.74). The results suggested that the single nucleotide polymorphisms Taq-1 t(T) allele and b (G allele) in Bsm-1 might be a susceptibility allele for diabetes in the Kashmiri population. CONCLUSIONS: VDR gene polymorphisms appear to be an important genetic determinant in the progression of diabetes. Considering the important predisposition risk factor, we observed that Taq-1 and Bsm-1 were strongly associated with diabetes in northern Indians. But requires further study as a probable genetic risk marker for diabetes.
OBJECTIVES: Approx 1 billion people across various ethnic and age groups have vitamin D deficiency. The high prevalence of such a deficiency is an imperative public health issue because hypovitaminosis D is an autonomous risk factor for mortality in the general population. Beyond bone integrity and calcium homeostasis, it is involved in numerous physiologic and pathologic processes. The role of vitamin D in the pathogenesis and prevention of type 2 diabetes mellitus has sparked universal interest. METHODS: This hospital-based case-control study was designed to study the association between 25-hydroxy vitamin D (25[OH]D) levels and the vitamin D receptor (VDR) gene polymorphism with diabetes and to evaluate their roles as risk factors for diabetes. 100 cases and controls were taken. 25(OH)D levels were analyzed by the chemilumenescence method using a Siemens ADVIA Centaur analyzer. Genomic DNA was extracted and Taq-1 and Bsm-1 genotyping in the VDR gene was done by using the polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP). RESULTS: 25(OH)D levels of patients with diabetes were significantly lower than those of controls (19.26±0.95 ng/mL vs. 25.49±1.02 ng/mL; p=0.001). 25(OH)D levels were found to be inversely associated with glycated hemoglobin percentages in cases (r2=0.74). The results suggested that the single nucleotide polymorphisms Taq-1 t(T) allele and b (G allele) in Bsm-1 might be a susceptibility allele for diabetes in the Kashmiri population. CONCLUSIONS:VDR gene polymorphisms appear to be an important genetic determinant in the progression of diabetes. Considering the important predisposition risk factor, we observed that Taq-1 and Bsm-1 were strongly associated with diabetes in northern Indians. But requires further study as a probable genetic risk marker for diabetes.
Keywords:
chemiluminescence; chimiluminescence; diabète de type 2; genetic polymorphisms; polymerase chain reaction; polymorphismes de longueur des fragments de restriction; polymorphismes génétiques; restriction fragment length polymorphisms; réaction en chaîne par polymérase; récepteur de la vitamine D; type2 diabetes; vitamin D receptor