Literature DB >> 28736500

Comparative Proteomic Analysis of Three Chinese Hamster Ovary (CHO) Host Cells.

Ningning Xu1, Chao Ma1, Jianfa Ou1, Wanqi Wendy Sun2, Lufang Zhou3, Hui Hu4, Xiaoguang Margaret Liu1.   

Abstract

Chinese hamster ovary (CHO) cells have been widely used to express heterologous genes and produce therapeutic proteins in biopharmaceutical industry. Different CHO host cells have distinct cell growth rates and protein expression characteristics. In this study, the expression of about 1,307 host proteins in three sublines, i.e. CHO K1, CHO S and CHO/dihydrofolate reductase (dhfr)- , were investigated and compared using proteomic analysis. The proteins involved in cell growth, glycolysis, tricarboxylic acid cycle, transcription, translation and glycosylation were quantitated using Liquid chromatography tandem-mass spectrometry (LC-MS/MS). The key host cell proteins that regulate the kinetics of cell growth and the magnitude of protein expression levels were identified. Furthermore, several rational cell engineering strategies on how to combine the desired features of fast cell growth and efficient production of therapeutic proteins into one new super CHO host cell have been proposed.

Entities:  

Keywords:  CHO sublines; cell growth; comparative proteomics; protein expression; regulators

Year:  2017        PMID： 28736500      PMCID： PMC5518618          DOI： 10.1016/j.bej.2017.05.007

Source DB:  PubMed          Journal:  Biochem Eng J        ISSN： 1369-703X            Impact factor:   3.978


  40 in total

1.  Increased filamin binding to beta-integrin cytoplasmic domains inhibits cell migration.

Authors:  D A Calderwood; A Huttenlocher; W B Kiosses; D M Rose; D G Woodside; M A Schwartz; M H Ginsberg
Journal:  Nat Cell Biol       Date:  2001-12       Impact factor: 28.824

Review 2.  GRP94: An HSP90-like protein specialized for protein folding and quality control in the endoplasmic reticulum.

Authors:  Michal Marzec; Davide Eletto; Yair Argon
Journal:  Biochim Biophys Acta       Date:  2011-11-03

3.  The genetics of somatic mammalian cells.

Authors:  T T PUCK
Journal:  Adv Biol Med Phys       Date:  1957

4.  Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells.

Authors:  Lianchun Fan; Ibrahim Kadura; Lara E Krebs; Christopher C Hatfield; Margaret M Shaw; Christopher C Frye
Journal:  Biotechnol Bioeng       Date:  2011-11-21       Impact factor: 4.530

5.  Identification of autocrine growth factors secreted by CHO cells for applications in single-cell cloning media.

Authors:  U Ming Lim; Miranda Gek Sim Yap; Yoon Pin Lim; Lin-Tang Goh; Say Kong Ng
Journal:  J Proteome Res       Date:  2013-06-25       Impact factor: 4.466

6.  Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.

Authors:  F T Kao; T T Puck
Journal:  Proc Natl Acad Sci U S A       Date:  1968-08       Impact factor: 11.205

7.  Comparative proteomics analysis of high n-butanol producing metabolically engineered Clostridium tyrobutyricum.

Authors:  Chao Ma; Kyoko Kojima; Ningning Xu; James Mobley; Lufang Zhou; Shang-Tian Yang; Xiaoguang Margaret Liu
Journal:  J Biotechnol       Date:  2014-11-05       Impact factor: 3.307

8.  Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases.

Authors:  Yolanda Santiago; Edmond Chan; Pei-Qi Liu; Salvatore Orlando; Lin Zhang; Fyodor D Urnov; Michael C Holmes; Dmitry Guschin; Adam Waite; Jeffrey C Miller; Edward J Rebar; Philip D Gregory; Aaron Klug; Trevor N Collingwood
Journal:  Proc Natl Acad Sci U S A       Date:  2008-03-21       Impact factor: 11.205

Review 9.  Novel functions of vimentin in cell adhesion, migration, and signaling.

Authors:  Johanna Ivaska; Hanna-Mari Pallari; Jonna Nevo; John E Eriksson
Journal:  Exp Cell Res       Date:  2007-04-14       Impact factor: 3.905

10.  Regulated overexpression of the survival factor bcl-2 in CHO cells increases viable cell density in batch culture and decreases DNA release in extended fixed-bed cultivation.

Authors:  M Fussenegger; D Fassnacht; R Schwartz; J A Zanghi; M Graf; J E Bailey; R Pörtner
Journal:  Cytotechnology       Date:  2000-01       Impact factor: 2.058
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  5 in total

1.  Development of a stable antibody production system utilizing an Hspa5 promoter in CHO cells.

Authors:  Hiroki Tanemura; Kenji Masuda; Takeshi Okumura; Eri Takagi; Daisuke Kajihara; Hirofumi Kakihara; Koichi Nonaka; Ryo Ushioda
Journal:  Sci Rep       Date:  2022-05-24       Impact factor: 4.996

2.  Non-toxic fragment of botulinum neurotoxin type A and monomethyl auristatin E conjugate for targeted therapy for neuroendocrine tumors.

Authors:  Jason Whitt; Won S Hong; Rahul R Telange; Chee Paul Lin; James Bibb; David J Beebe; Herbert Chen; Renata Jaskula-Sztul
Journal:  Cancer Gene Ther       Date:  2020-02-07       Impact factor: 5.987

3.  Untargeted proteomics reveals upregulation of stress response pathways during CHO-based monoclonal antibody manufacturing process leading to disulfide bond reduction.

Authors:  Seo-Young Park; Susan Egan; Anthony J Cura; Kathryn L Aron; Xuankuo Xu; Mengyuan Zheng; Michael Borys; Sanchayita Ghose; Zhengjian Li; Kyongbum Lee
Journal:  MAbs       Date:  2021 Jan-Dec       Impact factor: 5.857

4.  A comprehensive CHO SWATH-MS spectral library for robust quantitative profiling of 10,000 proteins.

Authors:  Kae Hwan Sim; Lillian Chia-Yi Liu; Hwee Tong Tan; Kelly Tan; Daniel Ng; Wei Zhang; Yuansheng Yang; Stephen Tate; Xuezhi Bi
Journal:  Sci Data       Date:  2020-08-11       Impact factor: 6.444

5.  Bioprocess development of antibody-drug conjugate production for cancer treatment.

Authors:  Jianfa Ou; Yingnan Si; KahYong Goh; Norio Yasui; Yichen Guo; Jiajia Song; Lizhong Wang; Renata Jaskula-Sztul; Jinda Fan; Lufang Zhou; Runhua Liu; Xiaoguang Liu
Journal:  PLoS One       Date:  2018-10-23       Impact factor: 3.240
  5 in total

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