BACKGROUND: Differential gene expression in peripheral blood mononuclear cells (PBMCs) after Roux-en-Y gastric bypass (RYGB) is poorly characterized. Markers of these processes may provide a deeper understanding of the mechanisms that underlie these events. The main goal of this study was to identify changes in PBMC gene expression in women with obesity before and 6 months after RYGB-induced weight loss. METHODS: The ribonucleic acid (RNA) of PBMCs from 13 obese women was analyzed before and 6 months after RYGB; the RNA of PBMCs from nine healthy women served as control. The gene expression levels were determined by microarray analysis. Significant differences in gene expression were validated by real-time quantitative polymerase chain reaction (RT-qPCR). RESULTS: Microarray analysis for comparison of the pre- and postoperative periods showed that 1366 genes were differentially expressed genes (DEGs). The main pathways were related to gene transcription; lipid, energy, and glycide metabolism; inflammatory and immunological response; cell differentiation; oxidative stress regulation; response to endogenous and exogenous stimuli; substrate oxidation; mTOR signaling pathway; interferon signaling; mitogen-activated protein kinases (MAPK), cAMP response element binding protein (CREB1), heat shock factor 1 (HSF1), and sterol regulatory element binding protein 1c (SREBP-1c) gene expression; adipocyte differentiation; and methylation. CONCLUSIONS: Six months after bariatric surgery and significant weight loss, many molecular pathways involved in obesity and metabolic diseases change. These findings are an important tool to identify potential targets for therapeutic intervention and clinical practice of nutritional genomics in obesity.
BACKGROUND: Differential gene expression in peripheral blood mononuclear cells (PBMCs) after Roux-en-Y gastric bypass (RYGB) is poorly characterized. Markers of these processes may provide a deeper understanding of the mechanisms that underlie these events. The main goal of this study was to identify changes in PBMC gene expression in women with obesity before and 6 months after RYGB-induced weight loss. METHODS: The ribonucleic acid (RNA) of PBMCs from 13 obesewomen was analyzed before and 6 months after RYGB; the RNA of PBMCs from nine healthy women served as control. The gene expression levels were determined by microarray analysis. Significant differences in gene expression were validated by real-time quantitative polymerase chain reaction (RT-qPCR). RESULTS: Microarray analysis for comparison of the pre- and postoperative periods showed that 1366 genes were differentially expressed genes (DEGs). The main pathways were related to gene transcription; lipid, energy, and glycide metabolism; inflammatory and immunological response; cell differentiation; oxidative stress regulation; response to endogenous and exogenous stimuli; substrate oxidation; mTOR signaling pathway; interferon signaling; mitogen-activated protein kinases (MAPK), cAMP response element binding protein (CREB1), heat shock factor 1 (HSF1), and sterol regulatory element binding protein 1c (SREBP-1c) gene expression; adipocyte differentiation; and methylation. CONCLUSIONS: Six months after bariatric surgery and significant weight loss, many molecular pathways involved in obesity and metabolic diseases change. These findings are an important tool to identify potential targets for therapeutic intervention and clinical practice of nutritional genomics in obesity.
Authors: Armen Khatchadourian; Simon D Bourque; Vincent R Richard; Vladimir I Titorenko; Dusica Maysinger Journal: Biochim Biophys Acta Date: 2012-01-28
Authors: Kamal K Mahawar; Parveen Kumar; Chetan Parmar; Yitka Graham; William R J Carr; Neil Jennings; Norbert Schroeder; Shlok Balupuri; Peter K Small Journal: Obes Surg Date: 2016-03 Impact factor: 4.129
Authors: Sérgio D J Pena; Giuliano Di Pietro; Mateus Fuchshuber-Moraes; Julia Pasqualini Genro; Mara H Hutz; Fernanda de Souza Gomes Kehdy; Fabiana Kohlrausch; Luiz Alexandre Viana Magno; Raquel Carvalho Montenegro; Manoel Odorico Moraes; Maria Elisabete Amaral de Moraes; Milene Raiol de Moraes; Elida B Ojopi; Jamila A Perini; Clarice Racciopi; Andrea Kely Campos Ribeiro-Dos-Santos; Fabrício Rios-Santos; Marco A Romano-Silva; Vinicius A Sortica; Guilherme Suarez-Kurtz Journal: PLoS One Date: 2011-02-16 Impact factor: 3.240
Authors: Adil Mardinoglu; John T Heiker; Daniel Gärtner; Elias Björnson; Michael R Schön; Gesine Flehmig; Nora Klöting; Knut Krohn; Mathias Fasshauer; Michael Stumvoll; Jens Nielsen; Matthias Blüher Journal: Sci Rep Date: 2015-10-05 Impact factor: 4.379
Authors: Ravi V Shah; Shih-Jen Hwang; Ashish Yeri; Kahraman Tanriverdi; Alexander R Pico; Chen Yao; Venkatesh Murthy; Jennifer Ho; Olga Vitseva; Danielle Demarco; Sajani Shah; Mark D Iafrati; Daniel Levy; Jane E Freedman Journal: Arterioscler Thromb Vasc Biol Date: 2019-01 Impact factor: 8.311
Authors: Tammy Lo; Renuka S Haridas; Eleanor J M Rudge; Robert P Chase; Keyvan Heshmati; Elizabeth M Lucey; Alison M Weigl; Otatade J Iyoha-Bello; Chelsea O Ituah; Emily J Benjamin; Seth W McNutt; Leena Sathe; Leanna Farnam; Benjamin A Raby; Ali Tavakkoli; Damien C Croteau-Chonka; Eric G Sheu Journal: J Clin Endocrinol Metab Date: 2022-01-18 Impact factor: 6.134
Authors: Christoph Schwarz; Fabian Fitschek; David Bar-Or; Daniel A Klaus; Bianca Tudor; Edith Fleischmann; Georg Roth; Dietmar Tamandl; Thomas Wekerle; Michael Gnant; Martin Bodingbauer; Klaus Kaczirek Journal: PLoS One Date: 2017-10-18 Impact factor: 3.240
Authors: Marcela A S Pinhel; Natália Y Noronha; Carolina F Nicoletti; Vanessa Ab Pereira; Bruno Ap de Oliveira; Cristiana Cortes-Oliveira; Wilson Salgado; Fernando Barbosa; Júlio S Marchini; Doroteia Rs Souza; Carla B Nonino Journal: Int J Mol Sci Date: 2020-06-24 Impact factor: 5.923
Authors: C F Nicoletti; M A S Pinhel; A Diaz-Lagares; F F Casanueva; A Jácome; V C Pinhanelli; B A P de Oliveira; A B Crujeiras; C B Nonino Journal: BMC Med Genomics Date: 2019-05-27 Impact factor: 3.063
Authors: Andrea G Izquierdo; Marcos C Carreira; Gemma Rodriguez-Carnero; Alfredo Fernandez-Quintela; Aurelio M Sueiro; Miguel A Martinez-Olmos; German Guzman; Daniel De Luis; Marcela A S Pinhel; Carolina F Nicoletti; Carla B Nonino; Francisco J Ortega; Maria P Portillo; Jose M Fernandez-Real; Felipe F Casanueva; Ana B Crujeiras Journal: Int J Obes (Lond) Date: 2020-06-16 Impact factor: 5.095