Eleni Priglinger1, Christina M A P Schuh2, Carolin Steffenhagen2, Christoph Wurzer2, Julia Maier2, Sylvia Nuernberger3, Wolfgang Holnthoner2, Christiane Fuchs4, Susanne Suessner5, Dominik Rünzler4, Heinz Redl2, Susanne Wolbank2. 1. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Workers' Compensation Board (AUVA) Research Center, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria. Electronic address: eleni.priglinger@trauma.lbg.ac.at. 2. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Workers' Compensation Board (AUVA) Research Center, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria. 3. Austrian Cluster for Tissue Regeneration, Vienna, Austria; Bernhard Gottlieb University Clinic of Dentistry, Universitätsklinik für Zahn-, Mund- und Kieferheilkunde Ges.m.b.H, Vienna, Austria; Medical University of Vienna, Department of Trauma Surgery, Vienna, Austria. 4. Austrian Cluster for Tissue Regeneration, Vienna, Austria; University of Applied Sciences Technikum Wien, Department of Biochemical Engineering, Vienna, Austria. 5. Austrian Cluster for Tissue Regeneration, Vienna, Austria; Red Cross Blood Transfusion Service of Upper Austria, Linz, Austria.
Abstract
BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation.
BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency. METHODS: In our experimental set-up, low-energy extracorporeal shock wave therapy (ESWT) was performed on freshly obtained human adipose tissue and isolated adipose tissue SVF cells aiming to equalize and enhance stem cell properties and functionality. RESULTS: After ESWT on adipose tissue we could achieve higher cellular adenosine triphosphate (ATP) levels compared with ESWT on the isolated SVF as well as the control. ESWT on adipose tissue resulted in a significantly higher expression of single mesenchymal and vascular marker compared with untreated control. Analysis of SVF protein secretome revealed a significant enhancement in insulin-like growth factor (IGF)-1 and placental growth factor (PLGF) after ESWT on adipose tissue. DISCUSSION: Summarizing we could show that ESWT on adipose tissue enhanced the cellular ATP content and modified the expression of single mesenchymal and vascular marker, and thus potentially provides a more regenerative cell population. Because the effectiveness of autologous cell therapy is dependent on the therapeutic potency of the patient's cells, this technology might raise the number of patients eligible for autologous cell transplantation.
Authors: Carina Hromada; Jaana Hartmann; Johannes Oesterreicher; Anton Stoiber; Anna Daerr; Barbara Schädl; Eleni Priglinger; Andreas H Teuschl-Woller; Wolfgang Holnthoner; Johannes Heinzel; David Hercher Journal: Biomolecules Date: 2022-06-12
Authors: Michael Graber; Felix Nägele; Jakob Hirsch; Leo Pölzl; Victor Schweiger; Sophia Lechner; Michael Grimm; John P Cooke; Can Gollmann-Tepeköylü; Johannes Holfeld Journal: Front Cardiovasc Med Date: 2022-05-12
Authors: Cyrill Slezak; Roland Rose; Julia M Jilge; Robert Nuster; David Hercher; Paul Slezak Journal: Int J Mol Sci Date: 2021-12-28 Impact factor: 5.923
Authors: Helene Zirath; Mario Rothbauer; Sarah Spitz; Barbara Bachmann; Christian Jordan; Bernhard Müller; Josef Ehgartner; Eleni Priglinger; Severin Mühleder; Heinz Redl; Wolfgang Holnthoner; Michael Harasek; Torsten Mayr; Peter Ertl Journal: Front Physiol Date: 2018-07-03 Impact factor: 4.566