Jae-Woo Kwon1, Hun Soo Chang2, Jeong-Seok Heo2, Da-Jeong Bae2, Jong-Uk Lee2, Chang An Jung2, Ji-Hye Son2, Jong Sook Park3, Soo Hyun Kim4, Kyung-Up Min5, Choon-Sik Park6. 1. Division of Allergy and Clinical Immunology, Kangwon National University School of Medicine, Chuncheon, South Korea. 2. Department of Interdisciplinary Program in Biomedical Science Major, Soonchunhyang Graduate School, Bucheon, South Korea. 3. Division of Allergy and Respiratory Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, South Korea. 4. Laboratory of Cytokine Immunology, Department of Biomedical Science and Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701, South Korea. 5. Department of Internal Medicine, Seoul National University College of Medicine, Seoul, South Korea. 6. Division of Allergy and Respiratory Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, South Korea. Electronic address: mdcspark@daum.net.
Abstract
BACKGROUND: Interleukin-32(IL-32)γ is a pro-inflammatory cytokine involved in the development and severity of chronic inflammatory diseases, but its role in asthma is unclear. OBJECTIVE: This study was conducted to evaluate the relationship of IL-32γ levels in sputum with the severity of asthma. METHODS: IL-32γ levels in the supernatant of induced sputum obtained from 89 patients with stable asthma were measured using a sandwich enzyme-linked immunosorbent assay (ELISA). The relationships between sputum IL-32γ levels and baseline forced expiratory volume in 1 s (FEV1% pred.), inflammatory cell profiles in sputum, and annual frequency of asthma exacerbation were determined. RESULTS: IL-32γ was detected in the sputum of 25 of 89 (28.1%) asthma patients, and the levels of sputum were negatively correlated with FEV1% pred. (ρ = -0.312, p = 0.003). The annual exacerbation rate was significantly higher in this group than in the IL-32-negative group (n = 64) (p = 0.03). Sputum IL-32γ levels correlated well with the annual exacerbation rate (ρ = 0.261, p = 0.014), but there were no differences in the inflammatory cell profiles in the induced sputum of IL-32-positive and IL-32-negative patients. CONCLUSION: The level of IL-32γ in induced sputum may be associated with asthma severity and related with higher risk of asthma exacerbation.
BACKGROUND:Interleukin-32(IL-32)γ is a pro-inflammatory cytokine involved in the development and severity of chronic inflammatory diseases, but its role in asthma is unclear. OBJECTIVE: This study was conducted to evaluate the relationship of IL-32γ levels in sputum with the severity of asthma. METHODS:IL-32γ levels in the supernatant of induced sputum obtained from 89 patients with stable asthma were measured using a sandwich enzyme-linked immunosorbent assay (ELISA). The relationships between sputum IL-32γ levels and baseline forced expiratory volume in 1 s (FEV1% pred.), inflammatory cell profiles in sputum, and annual frequency of asthma exacerbation were determined. RESULTS:IL-32γ was detected in the sputum of 25 of 89 (28.1%) asthmapatients, and the levels of sputum were negatively correlated with FEV1% pred. (ρ = -0.312, p = 0.003). The annual exacerbation rate was significantly higher in this group than in the IL-32-negative group (n = 64) (p = 0.03). Sputum IL-32γ levels correlated well with the annual exacerbation rate (ρ = 0.261, p = 0.014), but there were no differences in the inflammatory cell profiles in the induced sputum of IL-32-positive and IL-32-negative patients. CONCLUSION: The level of IL-32γ in induced sputum may be associated with asthma severity and related with higher risk of asthma exacerbation.
Authors: Joo Hee Kim; Young Sook Jang; Hwan Il Kim; Ji Young Park; Sung Hoon Park; Yong Il Hwang; Seung Hun Jang; Ki Suck Jung; Hae Sim Park; Choon Sik Park Journal: Allergy Asthma Immunol Res Date: 2020-07 Impact factor: 5.764