What do we know about laminar connectivity?

In this brief review, I attempt an overview of the main components of anatomical laminar-level connectivity. These are: extrinsic outputs, excitatory and inhibitory intrinsic connectivity, and intrinsic inputs. Supporting data are biased from the visual system of nonhuman primates (NHPs), but I have drawn as much as possible from a broader span in order to treat the important issue of area-specific variability. In a second part, I briefly discuss laminar connectivity in the context of network organization (feedforward/feedback cortical connections, and the major types of corticothalamic connections). I also point out anatomical issues in need of clarification, including more systematic, whole brain coverage of tracer injections; more data on anterogradely labeled terminations; more complete, area-specific quantitative data about projection neurons, and quantitative data on terminal density and convergence. Postsynaptic targets are largely unknown, but their identification is essential for understanding the finer analysis and principles of laminar patterns. Laminar resolution MRI offers a promising new tool for exploring laminar connectivity: it is potentially fast and macro-scale, and allows for repeated investigation under different stimulus conditions. Conversely, anatomical resolution, although detailed beyond the current level of MRI visualization, offers a rich trove for experimental design and interpretation of fMRI activation patterns.