| Literature DB >> 28726498 |
Tatiane Roquete Amparo1, Ivanildes Vasconcelos Rodrigues2, Janaína Brandão Seibert1, Rafaella Hilda Zaniti Souza1, Amanda Ribeiro de Oliveira1, Vivette Appolinário Rodrigues Cabral3, Paula Melo de Abreu Vieira4, Geraldo Célio Brandão1, Adriana Akemi Okuma5, Sidney Augusto Vieira Filho1, Luiz Fernando Medeiros Teixeira1, Gustavo Henrique Bianco de Souza1.
Abstract
The crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3-80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against Staphylococcus saprophyticus. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC50). The results provide in vitro scientific support to the possible application of branches of P. spruceanum as antimicrobial agent that may contribute for treatment of infections.Entities:
Keywords: Protium spruceanum; antbacterial activity; cytotoxicity
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Year: 2017 PMID： 28726498 DOI： 10.1080/14786419.2017.1354182
Source DB: PubMed Journal: Nat Prod Res ISSN： 1478-6419 Impact factor: 2.861