| Literature DB >> 28726193 |
E G Skurikhin1, A V Pakhomova2, O V Pershina1, L A Ermolaeva1, V A Krupin1, N N Ermakova1, E S Pan1, A I Kudryashova1, O Yu Rybalkina1, T B Pavlovskaya1, N V Litvyakov3, V E Goldberg3, A M Dygai1.
Abstract
The properties of spermatogonial stem cells, endothelial progenitor cells, and the epithelial progenitors of C57Bl/6 mice under conditions of metabolic disorders were studied using the model of busulfan-induced suppression of spermatogenesis and in vitro culture technique. Spermatogonial stem cells CD117-CD90+ and epithelial progenitors CD45-CD31-Sca-1+CD49f+ derived from the testes of mice with metabolic disturbances demonstrated 17- and 28-fold increase in the respective cell mass and generated cell colonies in vitro. In contrast, spermatogonial stem cells with immune phenotype CD51-CD24+CD52+ had reduced selfrenewal capacity. Spermatogonial stem cells CD117-CD90+ and CD117+CD90+ as well as endothelial progenitors CD45-CD31+ derived from the testes of donor mice with metabolic disorders demonstrated high transplantation capacity in C57Bl/6 mouse testes damaged by cytostatic busulfan.Entities:
Keywords: endothelial progenitors; epithelial progenitors; inflammation; metabolic disturbances; spermatogonial stem cells
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Year: 2017 PMID： 28726193 DOI： 10.1007/s10517-017-3775-1
Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804