Literature DB >> 28725659

Explant Methodology for Analyzing Neuroblast Migration.

Kirsty J Dixon1, Alisa Turbic2, Ann M Turnley3, Daniel J Liebl4.   

Abstract

The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we describe the isolation of SVZ-derived RMS explants from the neonatal mouse brain, and the conditions required to culture and evaluate their migration.

Entities:  

Keywords:  Matrigel; Neural stem cells; Neuroblasts; Rostral migratory stream; SVZ-derived explants; in vitro

Year:  2017        PMID： 28725659      PMCID： PMC5512276          DOI： 10.21769/BioProtoc.2249

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  17 in total

1.  Investigations of neuronal migration in the central nervous system.

Authors:  Michael E Ward; Yi Rao
Journal:  Methods Mol Biol       Date:  2005

2.  Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.

Authors:  B A Reynolds; S Weiss
Journal:  Science       Date:  1992-03-27       Impact factor: 47.728

3.  A rapid, quantitative method for assessing axonal extension on biomaterial platforms.

Authors:  Jared M Cregg; Sherri L Wiseman; Nicole M Pietrzak-Goetze; Martyn R Smith; David B Jaroch; Daniel C Clupper; Ryan J Gilbert
Journal:  Tissue Eng Part C Methods       Date:  2010-04       Impact factor: 3.056

Review 4.  Architecture and cell types of the adult subventricular zone: in search of the stem cells.

Authors:  J M García-Verdugo; F Doetsch; H Wichterle; D A Lim; A Alvarez-Buylla
Journal:  J Neurobiol       Date:  1998-08

5.  The Rho kinase pathway regulates mouse adult neural precursor cell migration.

Authors:  Soo Yuen Leong; Clare H Faux; Alisa Turbic; Kirsty J Dixon; Ann M Turnley
Journal:  Stem Cells       Date:  2011-02       Impact factor: 6.277

6.  EphrinB3 restricts endogenous neural stem cell migration after traumatic brain injury.

Authors:  Kirsty J Dixon; Jose Mier; Shyam Gajavelli; Alisa Turbic; Ross Bullock; Ann M Turnley; Daniel J Liebl
Journal:  Stem Cell Res       Date:  2016-09-28       Impact factor: 2.020

7.  Cellular proliferation and migration following a controlled cortical impact in the mouse.

Authors:  Shilpa Ramaswamy; Gwen E Goings; Katherine E Soderstrom; Francis G Szele; Dorothy A Kozlowski
Journal:  Brain Res       Date:  2005-08-16       Impact factor: 3.252

8.  Reproducible expansion and characterization of mouse neural stem/progenitor cells in adherent cultures derived from the adult subventricular zone.

Authors:  Michelle H Theus; Jerome Ricard; Daniel J Liebl
Journal:  Curr Protoc Stem Cell Biol       Date:  2012-03

9.  A specialized vascular niche for adult neural stem cells.

Authors:  Masoud Tavazoie; Lieven Van der Veken; Violeta Silva-Vargas; Marjorie Louissaint; Lucrezia Colonna; Bushra Zaidi; Jose Manuel Garcia-Verdugo; Fiona Doetsch
Journal:  Cell Stem Cell       Date:  2008-09-11       Impact factor: 24.633

10.  The subventricular zone en-face: wholemount staining and ependymal flow.

Authors:  Zaman Mirzadeh; Fiona Doetsch; Kazunobu Sawamoto; Hynek Wichterle; Arturo Alvarez-Buylla
Journal:  J Vis Exp       Date:  2010-05-06       Impact factor: 1.355
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  2 in total

1.  Visualization of Rostral Migratory Stream in the Developing Rat Brain by In Vivo Electroporation.

Authors:  Yi-Wei Xie; Zhao-Yun Li; Jing Du; Yu Chen; Bing-Yu Chen; Tong-Tong Wang; Zhihui Huang; Shuangxing Hou; Ying Wang
Journal:  Cell Mol Neurobiol       Date:  2018-02-13       Impact factor: 5.046

2.  GPER1 Signaling Initiates Migration of Female V-SVZ-Derived Cells.

Authors:  Iris Haumann; Muriel Anne Sturm; Max Anstötz; Gabriele M Rune
Journal:  iScience       Date:  2020-04-18
  2 in total

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