| Literature DB >> 28717566 |
Wenyan Guo1,2, Xiuli Liu1,2, Yurong Liu1,2, Yadong Gang1,2, Xiaobin He3, Yao Jia1,2, Fangfang Yin1,2, Pei Li1,2, Fei Huang1,2, Hongfu Zhou1,2, Xiaojun Wang1,2, Hui Gong1,2, Qingming Luo1,2, Fuqiang Xu1,2,3, Shaoqun Zeng1,2.
Abstract
The pH-sensitive fluorescent proteins enabling chemical reactivation in resin are useful tools for fluorescence microimaging. EGFP or EYFP is good for such applications. For simultaneous two-color imaging, a suitable red fluorescent protein is an urgent need. Here a pH-sensitive red fluorescent protein, pHuji, is selected and verified to remain pH-sensitive in HM20 resin. We observe 183% fluorescence intensity of pHuji in resin-embeded mouse brain and 29.08-fold fluorescence intensity of reactivated pHuji compared to the quenched state. pHuji and EGFP can be quenched and chemically reactivated simultaneously in resin, thus enabling simultaneous two-color micro-optical sectioning tomography of resin-embedded mouse brain. This method may greatly facilitate the visualization of neuronal morphology and neural circuits to promote understanding of the structure and function of the brain.Entities:
Keywords: (110.4190) Multiple imaging; (160.2540) Fluorescent and luminescent materials; (170.3880) Medical and biological imaging; (180.2520) Fluorescence microscopy
Year: 2017 PMID： 28717566 PMCID： PMC5508827 DOI： 10.1364/BOE.8.003281
Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732