| Literature DB >> 28715766 |
Riccardo Torelli1, Margherita Cacaci1, Massimiliano Papi2, Francesco Paroni Sterbini1, Cecilia Martini1, Brunella Posteraro3, Valentina Palmieri4, Marco De Spirito5, Maurizio Sanguinetti1, Francesca Bugli1.
Abstract
E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.Entities:
Keywords: Alginate lyase; Biofilm; Deoxyribonuclease I; E. faecalis; E. faecium; Vancomycin
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Year: 2017 PMID： 28715766 DOI： 10.1016/j.colsurfb.2017.07.010
Source DB: PubMed Journal: Colloids Surf B Biointerfaces ISSN： 0927-7765 Impact factor: 5.268