| Literature DB >> 28714418 |
Jiejun Wu1, J Eric McDuffie1, Jiao Song1, Michael C Harris1, Holly Raymond1, Yafei Chen1, Leslie Nguyen1, Steven Nguyen1, Justin Kanerva1, Heather M McAllister1, Xiaohu Deng1, Neelakandha Mani1, Changlu Liu1, Siquan Sun1.
Abstract
We have developed a workflow to extract, separate, and semi-quantify bioactive oxysterols from mouse colon tissues and fecal matters using solid- and liquid-phase extractions, enzymatic and chemical modifications, and stable-isotope dilution LC/MS/MS. The method was applied to a dextran sodium sulphate (DSS)-induced mouse colitis model, which revealed that one particular dihydroxycholesterol (diOHC), 7α,25-diOHC, was significantly elevated in both colon tissue and fecal matters of mice with colitis compared to that in naïve mice. The extent of 7α,25-diOHC elevation was positively correlated with colitis severity. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.Entities:
Keywords: Dihydroxycholesterols; Mouse colitis biomarker.; Oxysterol analysis; Oxysterol quantification; Oxysterol separation
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Year: 2017 PMID： 28714418 DOI： 10.2174/1568026617666170713165519
Source DB: PubMed Journal: Curr Top Med Chem ISSN： 1568-0266 Impact factor: 3.295