Literature DB >> 28709898

Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures.

Denis Saulnier1, Sophie De Decker2, Delphine Tourbiez2, Marie Agnès Travers2.   

Abstract

To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.
Copyright © 2017 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Gene sequencing; Molluscs; Multiplex real-time PCR; Splendidus clade; Vibrio aestuarianus

Mesh：

Year:  2017        PMID： 28709898     DOI： 10.1016/j.mimet.2017.07.002

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  5 in total

1.  A European epidemiological survey of Vibrio splendidus clade shows unexplored diversity and massive exchange of virulence factors.

Authors:  H Nasfi; M A Travers; J de Lorgeril; C Habib; T Sannie; L Sorieul; J Gerard; J C Avarre; P Haffner; D Tourbiez; T Renault; D Furones; A Roque; C Pruzzo; D Cheslett; R Gdoura; T Vallaeys
Journal:  World J Microbiol Biotechnol       Date:  2015-01-14       Impact factor: 3.312

2.  Descriptions of Mikrocytos veneroïdes n. sp. and Mikrocytos donaxi n. sp. (Ascetosporea: Mikrocytida: Mikrocytiidae), detected during important mortality events of the wedge clam Donax trunculus Linnaeus (Veneroida: Donacidae), in France between 2008 and 2011.

Authors:  Céline Garcia; Christophe Haond; Bruno Chollet; Mirella Nerac; Emmanuelle Omnes; Jean-Pierre Joly; Christine Dubreuil; Delphine Serpin; Aimé Langlade; Dominique Le Gal; Aouregan Terre-Terrillon; Olivier Courtois; Benjamin Guichard; Isabelle Arzul
Journal:  Parasit Vectors       Date:  2018-03-02       Impact factor: 3.876

3.  A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks.

Authors:  M A Travers; Céline Garcia; M Moussa; E Cauvin; A Le Piouffle; O Lucas; A Bidault; C Paillard; F Benoit; B Thuillier; M Treilles
Journal:  Appl Microbiol Biotechnol       Date:  2021-02-15       Impact factor: 4.813

4.  Transcriptomic Response of Mussel Gills After a Vibrio splendidus Infection Demonstrates Their Role in the Immune Response.

Authors:  Amaro Saco; Magalí Rey-Campos; Beatriz Novoa; Antonio Figueras
Journal:  Front Immunol       Date:  2020-12-16       Impact factor: 7.561

5.  Vibrio aestuarianus subsp. cardii subsp. nov., pathogenic to the edible cockles Cerastoderma edule in France, and establishment of Vibrio aestuarianus subsp. aestuarianus subsp. nov. and Vibrio aestuarianus subsp. francensis subsp. nov.

Authors:  Céline Garcia; Aurélie Mesnil; Delphine Tourbiez; Mirna Moussa; Christine Dubreuil; Amélie Gonçalves de Sa; Bruno Chollet; Yoann Godfrin; Lionel Dégremont; Delphine Serpin; Marie-Agnès Travers
Journal:  Int J Syst Evol Microbiol       Date:  2021-02       Impact factor: 2.747
  5 in total

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