Erdal Uysal1, Mehmet Dokur2, Serdar Altınay3, Eyup İlker Saygılı4, Kadir Batcıoglu5, Mehmet S Ceylan6, Hatem Kazımoglu7, Burcin A Uyumlu8, Mehmet Karadag9. 1. a Department of General Surgery , Sanko University School of Medicine , Gaziantep , Turkey. 2. b Emergency, Dr. Necip Fazil City Hospital , Kahramanmaras , Turkey. 3. c Department of Pathology , Bakırköy Dr Sadi Konuk Health Application and Research Center , Istanbul , Turkey. 4. d Department of Biochemistry , Sanko University School of Medicine , Gaziantep , Turkey. 5. e Department of Biochemistry , Inonu University Faculty of Pharmacy , Malatya , Turkey. 6. f Department of Otolaryngology , Sanko Hospital , Gaziantep , Turkey. 7. g Department of Urology , Sanko Universitesi Tip Fakultesi , Gaziantep , Turkey. 8. h Faculty of Pharmacy , Inonu University , Malatya , Turkey. 9. i Biostatistic and Medical Informatics, Health Sciences Institute , Inonu University , Malatya , Turkey.
Abstract
PURPOSE: In our study, it was aimed to investigate the preventive effect of milrinone on renal damage in experimental controlled non-heart-beating donors (NHBDs) model. MATERIALS AND METHODS: Sixteen rats randomly divided into 2 groups, 8 rats in each were used. Group 1 was control, group 2 was milrinone group. Group 1 rats received 1.25 ml 0.09% NaCl intraperitoneally equivalent to the milrinone diluted volume. Group 2 rats were administered intraperitoneally with 0.5 mg/kg of milrinone 2 hours before cardiac arrest. After the cardiac arrest, left nephrectomy was applied to the rats. Malondialdehyde, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) activities, Caspase-3 (apoptotic index) and histopathological evaluation were performed in the tissues. RESULTS: In the milrinone group, the total injury score was significantly lower relative to the control group (p = 0.001). Caspase-3 staining was moderately strong in the control group but weaker in the milrinone group. Apoptotic index was significantly lower in the milrinone group compared to the control group (p = 0.001). In comparison between groups, SOD and GPx in the milrinone group was significantly higher than the control group (p = 0.008, p = 0.006). CONCLUSIONS: Milrinone has been shown to be effective in the prevention of tissue damage due to oxidative stress and inflammatory process in the renal of warm ischemia in the experimental NHBDs model and in protecting the renal. Milrinone increases antioxidant activity while reducing apoptosis. Systemic administration of milrinone prior to cardiac arrest may be beneficial. Administration of milrinone to the recipient in the perioperative period may contribute to donor function.
PURPOSE: In our study, it was aimed to investigate the preventive effect of milrinone on renal damage in experimental controlled non-heart-beating donors (NHBDs) model. MATERIALS AND METHODS: Sixteen rats randomly divided into 2 groups, 8 rats in each were used. Group 1 was control, group 2 was milrinone group. Group 1 rats received 1.25 ml 0.09% NaCl intraperitoneally equivalent to the milrinone diluted volume. Group 2 rats were administered intraperitoneally with 0.5 mg/kg of milrinone 2 hours before cardiac arrest. After the cardiac arrest, left nephrectomy was applied to the rats. Malondialdehyde, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) activities, Caspase-3 (apoptotic index) and histopathological evaluation were performed in the tissues. RESULTS: In the milrinone group, the total injury score was significantly lower relative to the control group (p = 0.001). Caspase-3 staining was moderately strong in the control group but weaker in the milrinone group. Apoptotic index was significantly lower in the milrinone group compared to the control group (p = 0.001). In comparison between groups, SOD and GPx in the milrinone group was significantly higher than the control group (p = 0.008, p = 0.006). CONCLUSIONS:Milrinone has been shown to be effective in the prevention of tissue damage due to oxidative stress and inflammatory process in the renal of warm ischemia in the experimental NHBDs model and in protecting the renal. Milrinone increases antioxidant activity while reducing apoptosis. Systemic administration of milrinone prior to cardiac arrest may be beneficial. Administration of milrinone to the recipient in the perioperative period may contribute to donor function.
Authors: Ilaria Cicalini; Barbara De Filippis; Nicola Gambacorta; Antonio Di Michele; Silvia Valentinuzzi; Alessandra Ammazzalorso; Alice Della Valle; Rosa Amoroso; Orazio Nicolotti; Piero Del Boccio; Letizia Giampietro Journal: Molecules Date: 2020-04-15 Impact factor: 4.411