Mehmet Akyuz1, Seyithan Taysi2, Elif Baysal3, Elif Demir4, Hilal Alkis5, Muslum Akan2, Habip Binici6, Zeynel Abidin Karatas7. 1. Department of Chemistry, Science and Art Faculty, Kilis 7 Aralık University, Kilis, Turkey. 2. Department of Medical Biochemistry, Gaziantep University, Medical School, Gaziantep, Turkey. 3. Department of Otolaryngology, Inonu University, Medical School, Malatya, Turkey. 4. Division of Biochemistry, Harran University, College of Health, Osmanbey Campus, Şanl?urfa, Turkey. 5. Department of Radiation Oncology, Gaziantep University, Medical School, Gaziantep, Turkey. 6. Department of Otolaryngology, Harran University, Medical School, Sanliurfa, Turkey. 7. Department of Otolaryngology, Gaziantep University, Medical School, Gaziantep, Turkey.
Abstract
BACKGROUND: The purpose of this study was to investigate the radioprotective effects of thymoquinone against radiation-induced damage in the salivary glands of rats exposed to total cranial gamma irradiation. METHODS: Thirty-two Sprague-Dawley rats were divided into 4 groups to test the radioprotective effectiveness of thymoquinone by intraperitoneal injection. An appropriate control group was also studied. Biochemical parameters in liver tissue of rats were determined by spectrophotometer. RESULTS: Glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), total (enzymatic plus nonenzymatic) superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), and superoxide dismutase (SOD) activities were significantly decreased, whereas xanthine oxidase, nitric oxide synthase activities, malondialdehyde, nitric oxide, and peroxynitrite levels were significantly increased in the irradiation group when compared to the control and sham control groups. CONCLUSION: Results showed that thymoquinone reduces oxidative and nitrosative stress parameters and has antioxidant effects and a free radical scavenging activity.
BACKGROUND: The purpose of this study was to investigate the radioprotective effects of thymoquinone against radiation-induced damage in the salivary glands of rats exposed to total cranial gamma irradiation. METHODS: Thirty-two Sprague-Dawley rats were divided into 4 groups to test the radioprotective effectiveness of thymoquinone by intraperitoneal injection. An appropriate control group was also studied. Biochemical parameters in liver tissue of rats were determined by spectrophotometer. RESULTS: Glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), total (enzymatic plus nonenzymatic) superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), and superoxide dismutase (SOD) activities were significantly decreased, whereas xanthine oxidase, nitric oxide synthase activities, malondialdehyde, nitric oxide, and peroxynitrite levels were significantly increased in the irradiation group when compared to the control and sham control groups. CONCLUSION: Results showed that thymoquinone reduces oxidative and nitrosative stress parameters and has antioxidant effects and a free radical scavenging activity.
Authors: Kimberly J Jasmer; Kristy E Gilman; Kevin Muñoz Forti; Gary A Weisman; Kirsten H Limesand Journal: J Clin Med Date: 2020-12-18 Impact factor: 4.964